ACTA AGRICULTURAE BOREALI-SINICA ›› 2009, Vol. 24 ›› Issue (2): 8-11. doi: 10.7668/hbnxb.2009.02.002

Special Issue: Grape

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Cold Acclimation Induce the Changes in the Subcellular Distribution of Small Heat Shock Protein 17.6 in Young Grape Leaves During Heat Stress

ZHANG Jun-huan1, HUANG Wei-dong2   

  1. 1. College of Food Science and Nutritional Engineering,China Agricultural University, Beijing 100083, China;
    2. Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100093, China
  • Received:2008-12-09 Published:2009-04-28

Abstract: In order to invest igate the protect ing role of small heat shock protein 17. 6( sHSP17. 6) in the heat tolerance induced by cold acclimat ion( CA),and then to explore the mechanism of cross adaptation to different temperature stresses in grape plants. Leaves from two-year-old young grape ( Vitis vinif era L. cv. Jingxiu) plants were used as exper-imental materials.The subcellular localizations of sHSP17. 6 within mesophyll cells were observed using immuno-gold labeling electron microscopic technique. The results showed that CA pretreatment could enhance the expression level of sHSP17. 6 within heat stress. Compared to non-acclimated plants, the density of sHSP17. 6 gold particles was obviously increased in the nucleus and chloroplast of the CA leaves, especially 3 h after heat stress. Which offered the cytological evidence of sHSP17. 6 was contributed to the heat tolerance induced by CA pretreatment in grape plants.

Key words: Young grape plants, Cold acclimation, sHSP17. 6, Immuno-gold electron microscopic localization

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Cite this article

ZHANG Jun-huan, HUANG Wei-dong. Cold Acclimation Induce the Changes in the Subcellular Distribution of Small Heat Shock Protein 17.6 in Young Grape Leaves During Heat Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(2): 8-11. doi: 10.7668/hbnxb.2009.02.002.

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