Construction of a cDNA Subtractive Library Enriched the Response Genes of Deficient-Pi Stress and Functional Identification of Some ESTs in the Library

doi:10.7668/hbnxb.2009.02.003

Abstract

Abstract: The responses of plant to deficient-Pistress on phenotype and biochemical levels are the distinctly cooperative results resulting from the specific expressed genes at the spatiotemporal range. In this study,using Shixin828,the wheat cultivar with high phosphorus utilization efficiency identified previously to be the experimental material,a root cDNA selected2subtractive suppression library enriched the response genes of deficient2Pi stress at various time-points was constructed.The clone number in total was 2682 in this library.It was found that the insertion lengths changed from 350 bp to 750 bp,by analyzing the randomly selected clones in the library.The clones with putative functions and the clones with unknown functions were comprised of the percentage of 70 % and 30 %,respectively.The former,being homogenous to wheat,barley,rice,maize,and Arabidopsis,owned the functions in the plant by involving in the transcriptional regulation,protein synthesis,and biochemical metabolism.Therefore,the root cDNA selected-subtractive suppression library will be useful for further identification of the gene2regulatory network to respond to the deficient-Pi and cloning of important genes related to forming the high-phosphorus utilization efficiency.

Key words: Wheat(Triticum aestivum L. ), Deficient-Pi stress, Specific expressed gene, cDNA selected-subtractive suppression library

CLC Number:

S12.03

LONG Su-xia, LU Wen-jing, GU Jun-tao, GUO Cheng-jin, XIAO Kai. Construction of a cDNA Subtractive Library Enriched the Response Genes of Deficient-Pi Stress and Functional Identification of Some ESTs in the Library[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(2): 12-16. doi: 10.7668/hbnxb.2009.02.003.

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