SSR Markers of a Common Wheat Germplasm N9659 with Powdery Mildew Resistance Gene

doi:10.7668/hbnxb.2008.03.039

Abstract

Abstract: A powdery mildew resistance gene,originating from wild emmer(Triticum dicoccoides Thell.)accession AS846,was transferred to a common wheat line N9659.N9659 was highly resistant to the prevailing Erysiphegraminisf sp tritici Race in Shaanxi Province.The genetic analysis of N9659 was done in order to determine the inheritance of powdery mildew resistance in N9659.The reciprocal crosses F₁ between N9659 and the susceptible cultivar Shaan160 was highly resistant to the powdery mildew,the ratio of resistant plants and susceptible plants in F₂ progeny fitted the expected 3 to 1.127 F₂ plants of the crosses Shaan 160 × N9659 were used for SSR analysis,3 of 208 wheat SSR primer pairs WMS67,WMS408 and WMS604 generated polymorphic DNA fragments between the resistant plants DNA pools and susceptible plants DNA pools.By analyzing the polymorphic markers in these segregating populations,the SSR locus Xgwm67,Xgwm408,and Xgwm604 located on chromosome 5B were found to be linked to Powdery mildew resistance gene with the estimated genetic distance of 6.7,9.0 and 17.3 cM.The resistance gene derived from the mother Aegilops umbelluata AS846,was different from the resistant genes reported now,so it was problely a new gene.

Key words: Triticum aestivum, Rowdery mildew, Resistance gene, Chromosomal location, SSR marker

CLC Number:

S512.1.02

SANG Li-qun, WANG Chang-you, WANG Qiu-ying, JI Wan-quan. SSR Markers of a Common Wheat Germplasm N9659 with Powdery Mildew Resistance Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(3): 162-165. doi: 10.7668/hbnxb.2008.03.039.

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