Molecular Identification of Root-knot Nematodes on Vegetables in Greenhouses of Tianjin

doi:10.3969/j.issn.1000-7091.2012.05.034

Abstract

Abstract: In order to identify the species of root-knot nematodes occurring on vegetables in greenhouses in Tianjin region and provide theoretical support for disease control,11 samples were collected from 7 counties.The RFLP for Mitochondrial DNA and the sequencing for ITS region of ribosomal DNA were applied to analyze the nematodes samples.The results showed that the species for all samples were identified as Meloidogyne incognita.

Key words: Vegetable, Root knot nematode, Mitochondrial, DNA RFLP ITS region

CLC Number:

S436.3

HUO Jian-fei, REN Wen-lai, LIU Chun-yan, WANG Yong, TIAN Tao, HAO Yong-juan, WANG Wan-li, WAN Jiu-xing. Molecular Identification of Root-knot Nematodes on Vegetables in Greenhouses of Tianjin[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(5): 179-183. doi: 10.3969/j.issn.1000-7091.2012.05.034.

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