Full-length Genomes and Phylogenetic Analysis of Three Japanese Encephalitis Virus Isolates from Swine in Henan

doi:10.3969/j.issn.1000-7091.2012.05.035

Abstract

Abstract: In order to learn more about the complete genome and molecular evolution regular of Japanese encephalitis virus(JEV) strains isolated from swine,the full-length genome sequences of three JEV isolates,namely as BSF.ZZ-1,BSF.ZZ-3 and CSF.XZ-2D isolated from swine were analyzed in this paper.The results show that the length of the complete genomes of BSF.ZZ-1,BSF.ZZ-3 and CSF.XZ-2D was 10 977,10 977,10 976 nt,respectively.There is an insert base of ＂G＂ at the 10 701 nt site in the genomes of both BSF.ZZ-1 and BSF.ZZ-3.Compared to the virulent strain SA14,the homologies of nucleotide and amino acid sequences of BSF.ZZ-1,CSF.XZ-2D and BSF.ZZ-3 are 99.4% and 99.0%,respectively.Compared to the isolates HW(HW1),HWe(HW2) and SH0601 from swine,the homologies of the present three isolates are all more than 98.0%.Phylogenetic analysises with other 30 reference isolates covering all five JEV genotypes indicated that BSF.ZZ-1,CSF.XZ-2D and BSF.ZZ-3 located in a same evolutionary branch,belonging to the genotype III.

Key words: Swine, JEV Complete genome, Phylogenetic analysis

CLC Number:

S852.65

YU Le-le, TENG Man, LUO Jun, CHI Jia-qi, YU Zu-hua, CHAI Shu-jun, LIANG Xiao-xiao, ZHANG Gai-ping, WANG Ai-ping. Full-length Genomes and Phylogenetic Analysis of Three Japanese Encephalitis Virus Isolates from Swine in Henan[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(5): 184-190. doi: 10.3969/j.issn.1000-7091.2012.05.035.

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