Analysis of Fusarium Species Diversity from Field Soil in Western Henan Province

doi:10.3969/j.issn.1000-7091.2012.02.038

Abstract

Abstract: It was analyzed the phylogenetic relationships among Fusarium strains for the soil diversity of Fusarium species in western Henan province． The species diversity on Fusarium of field soil in western Henan province?was analyzed by methods of morphological modern molecular biology． It was obtained nine species of Fusarium?through the identification． There are?F． oxysporum，F． semitectum，F． verticillioides，F． graminearum，F． proliferatum，F． acumininatum，F． equiseti，F． thapsinum and F． solani． Phylogenetic analysis showed that 18S rDNA-ITS and?EF-1α gene sequences showed homology and the genetic polymorphism． 18S rDNA-ITS gene sequence analysis?showed that F verticillioides，F． proliferatum and F． thapsinum which belonged to Liseola Fusarium fungi species?presenced high homology． The performance can not be correct to manifest the classification of the Fusarium species．?Elongation factor EF-1α gene sequences better demonstrated the relationship of the tested strains between the species level． It indicated that elongation factor EF-1α gene sequences exist more abundant species polymorphisms than?18S rDNA-ITS gene sequence． It can be more better to show the relationship between the level of Fusarium species．

Key words: Fusarium, Species diversity, Phylogenetic analysis

CLC Number:

S154.36

TANG Lin, ZHAO Hui, GAO Zeng-gui. Analysis of Fusarium Species Diversity from Field Soil in Western Henan Province[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(2): 202-206. doi: 10.3969/j.issn.1000-7091.2012.02.038.

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References

[1] Booth C． The genus Fusarium[M]． Kew:Commonwealth?Mycological Institute，1971．
[2] Kistler H C，Alabouvette C，Baayen R P，et al． Systematic?numbering of vegetative compatibility groups in the plant?pathogenic fungus Fusarium oxysporum[J]． Phytopathology，1999，88:30－32．
[3] 徐雍皋，陈利锋．小麦赤霉病防治理论研究与实践
[J]．南京:江苏科学技术出版社，1993．
[4] 黄以柱．略论豫西地区环境的变迁与对策[J]．河南大?学学报:自然科学版，1985，1:51－58．
[5] 周国强，张?青．豫西地区土地分异规律及合理利用?研究[J]．地域研究与开发，2004，23(4):126－128．
[6] 方中达．植病研究方法[M]． 3 版．北京:中国农业出版?社，1998．
[7] 梁建根，张炳欣，喻景权，等．黄瓜根际重要病原与其?拮抗菌消长规律的研究[J]．应用生态学报，2005，16?(5):911－914．
[8] 马云华，魏?珉，王秀峰．日光温室连作黄瓜根区微生?物区系及酶活性的变化[J]．应用生态学报，2004，15?(6):1005－1008．
[9] 高同春，叶钟音，王?梅，等．水稻旱育秧苗立枯病致病镰刀菌分离、鉴定及致病性测定[J]．中国水稻科?学，2001，15(4) :320－322．
[10] ?Leslie J F，Summerell B A． The Fusarium laboratory?manual[M]． Ames:Blackwell Publishing，2006．
[11] ?Nelson P E，Toussoun T A，Marasas W F O． Fusarium?species:an illustrated manual for identification[M]．?Harrisburg:Pennsylvania State University Press，1983．
[12] 陈鸿逵，王拱辰．浙江镰刀菌志[M]．杭州:浙江科学?技术出版社，1992．
[13] Liu D，Coloe S，Baird R，et al． Rapid mini-preparation of?fungal DNA for PCR[J]． Joumal of Clinical Microbiology，2000，38(1):471．
[14] ?Chen Y C，Eisner J D，Kattar M M，et al． Polymorphic?internal transcribed spacer region 1 DNA sequences identify medically important yeasts[J]． Journal of Clinical Microbiology，2001，39(11):4042－4051．
[15] ?O' Donnell K． Molecular phylogeny of the Nectria?haematococca-Fusarium solani species complex[J]．?Mycologia，2000，92:919－938．
[16] ?陈玉玺，张利平，吕志堂． 10 株镰刀菌 rDNA 内转录?间隔区(ITS)序列分析[J]．安徽农业科学，2008，36?(12):4886－4887．
[17] Geiser D M，Jimenez-Gasco M，Kang S，et al． FusariumID v． 1． 0:A DNA sequence database for identifying Fusarium[J]． European Joumal of Plant Pathology，2004, 110:473－479．
[18] Gerlaeh W，Nirenberg H I． The genus Fusarium-a pietorial atlas[M]． Berlin:Paul Parey，1982．
[19] ?Guadet J，Julien J，Lafay J F，et al． Phylogeny of some?Fusarium species，as determined by large-subunit rRNA?sequence comparison[J]． Molecular Biology ＆ Eolution，1989，6:227－242．
[20] ?O' Donnell K，Cigelnik E． Two divergent intragenomic?rDNA ITS2 types within a monophyletic lineage of the?fungus Fusarium are nonorthologous[J]． Molecular Phylogenetics and Evolution，1997，7:103－116．
[21] O' Donnell K，Kistler H C，Cigelnik E，et al． Multiple evolutionary origins of the fungus causing Panama disease?of banana:Concordant evidence from nuclear and mitochondrial gene genealogies[J]． Proc Natl Acad Sci?USA，1998，95:2044－2049．
[22] O' Donnell K，Ward T J，Geiser D M，et al． Genealogical?concordance between the mating type locus and seven?other nuclear genes suppores formal recognition of nine?phylogenetically distinct species within the Fusarium?graminearum clade[J]． Fungal Genetics and Biology, 2004，41:600－623．
[23] 何玉梅，张仁陟，张丽华，等．不同耕作措施对土壤真?菌群落结构与生态特征的影响[J]．生态学报，2007, 27(1):113－119．
[24] O' Donnell K，Cigelnik E，Nirenberg H I． Molecular systematics and phylogeography of the Gibberella fujikumi?species complex[J]． Mycologia，1998，90:465－493．
[25] ?Kristensen R，Torp M，Kosiak B，et al． Phylogeny and?toxigenic potential is correlated in Fusarium species as?revealed by partial translation elongation factor 1 alpha?gene sequences[J]． Mycological Research，2005，109?(2):173－186．?

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