Abstract: One pair of primers was designed according to duck interferon-gamma (IFN-γ) gene sequences published in GenBank, which was used to amplify the IFN-γ gene from total RNA extracted directly from Gushi duck splenocyte without induction of PHA by reverse transcription-polymerase chain reaction (RT-PCR). The gene fragment was then cloned and sequenced. The result showed that the full length of the amplified duck IFN-γ gene sequence was 515 bp, which includes a open-reading frame (495 bp) and encodes 164 amino acid residues. There are three potential N-glycosalation sites and five cysteines in the deduced amino acid sequence. Compared with the duck IFN-γ gene sequences registered in GenBank, the the nucleotide sequence of Gushi duck IFN-γ gene was the same as AF087134, AF100929 and AJ012254, and shared 99.6% homology with Peking duck IFN-γ gene. Based on the gene sequence comparison and phylogenetic tree analysis, the genus difference was found in IFN-γ gene, and the closer the genetic relationship, the higher the homology. This study paved the way for future study of biological function and application of duck IFN-γ gene.

Key words: Gushi duck, IFN-γgene, Cloning, Phylogenetic analysis

CLC Number: 

• Q78