Transformation of Wheat with BADH Through Gene Gun and Agrobacterium

doi:10.3321/j.issn:1000-7091.2005.05.001

Abstract

Abstract: Salinity is very severe environmental problem hampering crop production.BADH is an important gene for conferring osmotic stress tolerance to plants.In this study gene gun and Agrobacterium were employed for the delivery of BADH into callus of wheat.Bombardment of 2 933 calli derived from immature embryos resulted in regeneration of 45 plant-lets after selection on phosphinothricin(PPT) with net regeneration rate of 1.53%.PCR analysis confirmed the presence of BADH gene in five plants.Results of PPT-leaf painting conformed to PCR test.In case of Agrobacterium mediated transformation five plant-lets regenerated from inoculation of 1 968 calli derived from mature embryos but none was PCR positive.PCR test of calli from mature embryos exhibited the successful delivery and integration of transgene.Inoculation of 2 933 calli from immature embryos resulted in regeneration of 21 plant-lets.PCR evaluation of regenerated plant-lets indicated the presence of BADH in 5 plants only.Net regeneration rate for immature embryos(0.72%) was higher than that of mature embryos(0.25%).Transformed plants obtained through Agrobacterium and gene gun were able to thrive in 0.5% NaCl(w/w) without stunted growth and wilting as compared with check plants indicating the successful integration and expression of BADH.

Key words: Transformation, Wheat, Agrobacterium, Gene gun, BADH

CLC Number:

S512.01

RAZZAQ Abdul, ZHANG Yan-min, ZHAO He, MA Zhi-ying, GUO Bei-hai, WANG Hai-bo. Transformation of Wheat with BADH Through Gene Gun and Agrobacterium[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2005, 20(5): 1-9. doi: 10.3321/j.issn:1000-7091.2005.05.001.

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