A Method of Using Chromosome Microdissection to Obtain Gene Probe in Wheat

doi:10.3321/j.issn:1000-7091.1998.04.004

Abstract

Abstract: The 1D chromosome long arms of common wheat Gang 82-122 were microdisseted with the cell manipulation instrument,and HMW-GS1Dx5 gene 5'region 400bp sequence was obtained using PCR amplification.The method of getting plant genes by microdissecting and microcloning was established,which is particularly useful to obtain a specific gene probe for the further study.

Key words: Wheat, Chromosome, Microdissection, HMW-GS1Dx5

CLC Number:

S512.103

Tian Chai, Lu Yifan, Deng Jixian, Liu Guangtian. A Method of Using Chromosome Microdissection to Obtain Gene Probe in Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1998, 13(4): 20-25. doi: 10.3321/j.issn:1000-7091.1998.04.004.

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