Abstract:
F iv e isozyme system s, namely dehydrog enase ( M DH ) , alco ho l dehy drog enase( A D H ) , pho spho gluco mutase ( P G M ) , est erase iso zym e ( EST ) and isocit ric dehy drog enase( IDH ) in ex tr act s f rom cot yl edons of germ inat ed t omat o seeds w ere analysed by polyacry-lamide gel elect ro phoresis ( PA G E) . Poly morphisms w ere observed at t hree loci ( MD H-1,M DH-2, MD H-3) . T he purit y of F1 hybrids , Jiaf en 1, Jiaf en 2 and Jiafen 15, w as t est ed by utilizing M DH-1 loci as markers, and the r esult s co mpar ed fav orabl y w it h t hose obt ained fr omfield purit y ident ification.
Key words:
Elect rophoresis,
Isozyme,
Cultivar purity identification,
Tomoato,
F1 hybrids
CLC Number:
Li Li， Zheng Xiaoying， Ma Lianping. Application of Dehydrogenase Allozymes in Test on Genetic Purity of F1 Hybrids of Tomato[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1998, 13(3): 116-120. doi: 10.3321/j.issn:1000-7091.1998.03.023.