Analysis of Isozymes of Different Growing Stages of Hybrid Millet Zhang and Their Parents

doi:10.3969/j.issn.1000-7091.2012.z1.033

Abstract

Abstract: Three isozymes of esterase( EST) ， glutamate oxaloacetate transaminase( GOT) and glutamate dehydrogenase( GLD) were performed by means of polyacrylamide gel electrophoresis to study the formating mechanismof heterosis of Foxtail Millet using hybrid Millet Zhang No． 3 ， 5， 6 and their parents respectively． The results indicated that the isozyme bands of EST， GOT and GLD were 14， 7 and 1 respectively in hybrid Millet Zhang and theirparents． The tested individuals and parents differed in number， Rf， activity or width of the EST and GOT bands． Esterase isozyme bands type of hybrid Millets were mainly parental complementary， zymogram pattern of both GOT andGLD in hybrid Millets were same as their parents． There is a correlation between the isozymes of patterns of EST andGOT and the formation of heterosis in hybrid Millet Zhang．

Key words: Hybrid Millet Zhang, EST, GOT, GLD, Isozyme

CLC Number:

S515

GUO Xiu-lin, ZHANG Chun-feng, WANG Yun, LIU Zi-hui, ZHAO Zhi-hai. Analysis of Isozymes of Different Growing Stages of Hybrid Millet Zhang and Their Parents[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.3969/j.issn.1000-7091.2012.z1.033.

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