Cloning Gene and Promoter of CkF5H from Caragana korshinskii Kom.

doi:10.7668/hbnxb.2018.02.012

Abstract

Abstract: CkF5H gene related to lignin synthesis was cloned from Caragana korshinskii Kom., an important leguminous forage shrub in the arid desert region of Northwest China. The full length of the gene cDNA was 1 679 bp. A total of 3 495 bp gDNA sequences of CkF5H gene of Caragana korshinskii were obtained,which had two exons,one intron,encoded 520 amino acids,GenBank accession number HQ829862. And the promoter sequence of 1 550 bp CkF5H gene was cloned by chromosome walking technique. The response elements on the promoter sequence were analyzed. The results showed that there were circadian rhythm response elements,defense and stress response elements,gibberellin cis-acting elements. CkF5H was rich in secondary structure,α-spiral accounted for 39.81%,β-fold accounted for 15.96%,irregular curl accounted for 44.23%,and predicted its three-dimensional structure.The predicted deduced amino acid sequence of CkF5H gene showed that the protein had an isoelectric point of 6.45,a molecular weight of 58.09 ku and an unstable coefficient of 45.72,which was an unstable protein with a total average hydrophilicity of -0.143 and a hydrophilic protein. Therefore,Cloning Caragana korshinskii Kom. CkF5H gene and detailed analysis will provide a theoretical basis for further study of the gene.

Key words: Caragana korshinskii Kom., F5H, Gene cloning, Lignin, RACE

CLC Number:

Q943.2

REN Fangyuan, LI Gao, ZHANG Tao, YANG Qi, WANG Ruigang. Cloning Gene and Promoter of CkF5H from Caragana korshinskii Kom.[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(2): 79-86. doi: 10.7668/hbnxb.2018.02.012.

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References

[1] Boerjan W,Ralph J,Baucher M.Lignin biosynthesis[J].Annu Rev Plant Biol,2003,54:519-546.
[2] Weng J K,Chapple C.The origin and evolution of lignin biosynthesis[J].The New Phytologist,2010,187(2):273-285.
[3] Vanholme R,Morreel K,Ralph J,et al.Lignin engineering[J].Current Opinion in Plant Biology,2008,11(3):278-285.
[4] Tran-Nguyen Nguyen,SeungHyun Son,Mark C,et al.Lignin biosynthesis in wheat (Triticum aestivum L.):its response to waterlogging and association with hormonal levels[J].BMC Plant Biology,2016,16(1):28.
[5] 王轶男,陈雪,盖颖,等.毛白杨木质素合成酶基因F5H克隆与生物信息学分析[J].广东农业科学,2014,20(2):131-140.
[6] Anderson Nickolas A,Tobimatsu Yuki,Ciesielski Peter N,et al.Manipulation of guaiacyl and syringyl monomer biosynthesis in an Arabidopsis cinnamyl alcohol dehydrogenase mutant results in atypical lignin biosynthesis and modified cell wall structure[J].The Plant Cell,2015,8(11):2195-2209.
[7] Robert W Sykes,Erica L Gjersing,Kirk Foutz,et al.Down-regulation of p-coumaroyl quinate/shikimate 3'-hydroxylase (C3'H) and cinnamate 4-hydroxylase (C4H) genes in the lignin biosynthetic pathway of Eucalyptus urophylla×E.grandis leads to improved sugar release[J].Biotechnology for Biofuels,2015,8(1):128.
[8] Faraji Mojdeh,Fonseca L L,Escamilla-Trevi n o Luis,et al.Computational inference of the structure and regulation of the lignin pathway in Panicum virgatum[J].Biotechnology for Biofuels,2015,8(1):151.
[9] Rencoret Jorge,Kim Hoon,Evaristo Anderson B,et al.Variability in lignin composition and structure in cell walls of different parts of Macaúba (Acrocomia aculeata) Palm Fruit[J].Journal of Agricultural and Food Chemistry,2018,66(1):138-153.
[10] Weng J K,Akiyama T,Bonawitz N D,et al.Convergent evolution of syringyl lignin biosynthesis via distinct pathways in the lycophyte Selaginella and flowering plants[J].The Plant Cell,2010,22(4):1033-1045.
[11] Liu J,Kim J I,Cusumano J C,et al.The impact of alterations in lignin deposition on cellulose organization of the plant cell wall[J].Plant Journal,2016,9(1):126.
[12] Ana Alves,Sara Santos,Rita Sim o es,et al.Characterization of residual lignin in cellulose isolated by the diglyme method from three Pinus species by IR spectroscopy and analytical pyrolysis[J].Holzforschung,2018,72(2):91-96.
[13] 曹山.毛果杨木质素形成相关基因克隆及功能研究[M].北京:北京林业大学,2016.
[14] 高玉寒,姚云峰,郭月峰,等.柠条锦鸡儿细根表面积密度对土壤水分空间分布的响应[J].中国农业科学,2017,33(5):136-142.
[15] 王赞,李源,陆景伟,等.中国柠条锦鸡儿资源表型多样性研究[J].华北农学报,2008,23(S):59-61.
[16] 高宇,张晓霞,李彬,等.国内外旱作农业研究进展[J].北方农业学报,2016,44(1):102-108.
[17] Arnold K,Bordoli L,Kopp J,et al.The SWISS-MODEL workspace:a web-based environment for protein structure homology modelling[J].Bioinformatics,2006,22(2):195-201.
[18] 郑晟,毛玉珊,张腾国,等.柠条锦鸡儿F3H基因克隆及功能分析[J].广西植物,2017,37(6):723-733.
[19] Reddy M S,Chen F,Shadle G,et al.Targeted down-regulation of cytochrome P450 enzymes for forage quality improvement in alfalfa (Medicago sativa L.)[J].Proceedings of the National Academy of Sciences of the United States of America,2005,102(46):16573-16578.
[20] Kim J H,Yang D H,Kim J S,et al.Cloning,characterization,and expression of two c DNA clones for arice ferulate-5-hydroxylase gene a cytochrome P450-receptors[J].Genest Cells,2000,5(1):57-69.
[21] Bewg W P,Poovaiah C,Wu L,et al.RNAi downregulation of three key lignin genes in sugarcane improves glucose release without reduction in sugar production[J].Biotechnology for Biofuels,2016,9(1):270.
[22] Tamura K,Peterson D,Peterson N,et al.MEGA5:molecular evolutionary genetics analysis using maximum likelihood,evolutionary distance,and maximum parsimony methods[J].Molecular Biology and Evolution,2011,28(10):2731-2739.
[23] Moura J C,Bonine C A,De Oliveira Fernandes Viana J,et al.Abiotic and biotic stresses and changes in the lignin content and composition in plants[J].Journal of Integrative Plant Biology,2010,52(4):360-376.
[24] Faraji M,Fonseca L L,Escamilla-Trevi n o L,et al.Computational inference of the structure and regulation of the lignin pathway in Panicum virgatum[J].Biotechnology for Biofuels,2015,8(1):151.
[25] Farhana Shafrin,Sudhanshu Sekhar Das,Neeti Sanan-Mishra,et al.Artificial miRNA-mediated down-regulation of two monolignoid biosynthetic genes (C3H and F5H) cause reduction in lignin content in jute[J].Plant Molecular Biology,2015,89(4-5):511-527.
[26] JoséRenán García,Nickolas Anderson,Regis Le-Feuvre,et al.Rescue of syringyl lignin and sinapate ester biosynthesis in Arabidopsis thaliana by a coniferaldehyde 5-hydroxylase from Eucalyptus globulus[J].Plant Cell,2014,33(8):1263-1274.
[27] 郭晓艺,胡尚连,曹颖,等.调控S木质素合成基因F5H1研究进展及对竹遗传改良的展望[J].福建林业科技,2007,34(3):234-237,243.

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