The HFW-05 Strains of Beauveria bassiana in Persistent Study on Vegetables

doi:10.7668/hbnxb.2015.S1.061

Abstract

Abstract: This paper studied using conventional foliar spray HFW-05 strain vegetables on persistent condition. Before applying Bassiana fungus,bacteria and actinomycetes background was investigated.It used 2.5×10⁷ spores/mL concentration for three consecutive times,each time interval 5 d.The results showed Beauveria bassiana on different host plants into colonies wavy within two months.On the surface of different crop leaves, Beauveria bassiana eventually reached 10⁶cfu/g in fresh leaves eventually persistent stock level.The final persistent quantity of Beauveria bassiana in mesophyll of fresh leaf level reached 10³cfu/g.Meanwhile,the environmental release of Beauveria bacteria,actinomyces quantitative impact was not obvious,does not destroy the original ecological balance.

Key words: Beauveria bassiana, Tomato, Cabbage, Persistent

CLC Number:

S476.12

SONG Jian, CAO Wei-ping, ZHANG Hai-jian, WANG guang-jun, DU Li-xin. The HFW-05 Strains of Beauveria bassiana in Persistent Study on Vegetables[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(S1): 336-341. doi: 10.7668/hbnxb.2015.S1.061.

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http://www.hbnxb.net/EN/Y2015/V30/IS1/336

References

[1] St Leger R J,Screen S.Prospects for strain improvement of fungal pathogens of insects and weeds[J].Fungal as Biocontrol Agents.London:CABI Publish House,2001:219-237.
[2] Blake R,Bextun E,Harlan G,et al.Field applications of bait-formulated Beauveria bassiana alginate pellets for biological control of the red imported fire ant[J].Biological Control,2002,31(4):746-752.
[3] Felipet,Marioz,Raquela,et al.Pathogenicity of Beauveria bassiana[J].Florida Entomol,2004,87(4):533-539.
[4] Furlong M J,Groden E.Evaluation of synergistic interactions between the Colorado potato Beetle(Coleoptera:Chrysomelidae) pathogen Beauveria bassiana and the insecticides,imidacloprid,and cyromazine[J].Journal of Economic Entomology,2001,94(2):344-356.
[5] Chen B,Feng M.Evaluation of interactive efficacy of two mycoinsecticides and low application rate imidacloprid in controlling greenhouse whitefly Trialeurodes vaporariorum (Homoptera:Aleyrodidae)[J].The Journal of Applied Ecology,2003,14(11):1934-1938.
[6] 姜怡,杨颖,陈华红,等.植物内生菌资源[J].微生物学通报,2005,32(6):146-147.
[7] Strobel G,Stierle A,Stierle D.Taxomyces a ndreana:A proposed new taxon for a bulbilli ferous by phomycete associated with pacific yew(Taxus brevifolia)[J].Mycotoxon,1993,40(7):71-81.
[8] 林坤.植物内生性白僵菌在病害防治中的作用[J].吉林农业,2011(3):105.
[9] Griffin M R.Beauveria bassiana,a cotton endophyte with biocontrol activity against seeding disease[M].Knoxville:The University of Tennessee,2007.
[10] 曹伟平,王金耀,冯书亮,等.球孢白僵菌HFW-05的诱变筛选及其对烟粉虱若虫的毒力测定[J].中国生物防治,2007,23(2):133-137.
[11] 秦长生.保水剂对白僵菌发芽率影响的初步研究[J].广东林业科技,2003,19(4):30-32.
[12] Luz C,Fargues J.Temperature and moisture requirements for conidial germination of an isolate of Beauveria bassiana,Pathogenic to Rhodnius prolixus.[J] Mycopathologia,1997,138:117-125.
[13] Lazzarini G M,Rocha L F,Luz C.Impact of moisture on in vitro germination of Metarhizium anisopliae and Beauveria bassiana and their activity on Triatoma infestans[J].Mycological Research,2006,110(4):485-492.
[14] Iskandarov U S,Guzalova A G,Davranov K D.Effects of nutrient medium composition and temperature on the germination of conidia and the entomopathogenic activity of the fungi[J].Prikladnaia Biokhimiiai Mikrobiologiia,2006,42(1):81-85.
[15] 武觑文.微生物杀虫剂的应用-松毛虫综合管理[M].北京:中国林业出版社,1990:256-273.
[16] 邝灼彬,吕利华,冯夏,等.温度及常见农药对球孢白僵菌生物学特性的影响[J].华南农业大学学报,2005,26(3):26-29.
[17] Lingg A J,Donaldson M D.Biotic and abiotic factors affecting stability of Beauveria bassiana conidia in soil[J].Journal of Invertebrate Pathology,1981,38(2):191-200.
[18] 何恒果.高毒效杀蚜球孢白僵菌菌株筛选[J].中国生态农业学报,2007,15(3):113-116.
[19] He Y R,Lu L H,Kuang Z B,et al.Effect of temperature and humidity on the virulence of beetle-derived Beauveria bassiana (Balsamo)Vuillemin(Deuteromycetes:Moniliales)against the daikon leaf Beetle,Phaedon brassicae Baly(Coleoptera:Chrysomelidae)[J].Acta Entomologica Sinica,2005,48(5):679-686.
[20] Bello D,Padin G,Juarez S,et al.Biocontrol of Acanthoscelides obtectus and Sitophilus oryzae with diatomaceous earth and Beauveria bassiana on stored grains[J].Biocontrol Science and Technology,2006,16(1/2):215-220.
[21] Vassilakos T N,Athanassiou C G,Kavallieratos N G,et al.Influence of temperature on the insecticidal effect of Beauveria bassiana in combination with diatomaceous earth against Rhyzopertha dominica and Sitophilus oryzae on stored wheat[J].Biological Control,2006,38(2):270-281.
[22] 王成树,王四宝,樊美珍,等.球孢白僵菌菌株耐热力与贮藏稳定性的关系[J].中国生物防治,1999,15(4):162-165.
[23] 王成树,李增智,樊美珍.球孢白僵菌孢子紫外照射后的光复活现象[J].菌物系统,1998,17(4):93-95.
[24] Ignoffo C M,Garcia C.Influence of conidial color on inactivation of several entomogenous fungi(Hyphomycetes) by stimulated sunlight[J].Environ Entomol 1992,21:913-917.
[25] Inglis G D,Johnson D L,Goettel M S.Effects of simulated rain on the persistence of Beauveria bassiana on leaves of alfalfa and wheat[J].Biocontr Sci Technol 1995,5:365-369.
[26] Edgington S,Segura H,De La Rosa W,et al.Photoprotection of Beauveria bassiana :testing simple formulations for control of the coffee berry borer[J].International Journal of Pest Management,2000,46(3):169-176.

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