Cloning and Expression Analysis of LPAT5 from Brassica napus

doi:10.7668/hbnxb.2014.05.007

Abstract

Abstract: In order to further study on biological function of BnLPAT5,we cloned and analysed this gene for its temporal and spatial expression and stress expression.Combined with the technology of homology-based cloning and semi-quantitative RT-PCR,one copy of LPAT5 full-length CDs sequence(1 041 bp)had been cloned from Brassica napus of Xiangyou 15 in our study,and designated as BnLPAT5.Sequence analysis revealed that BnLPAT5 had the LPLAT_LCLAT1 like domain and belonged to the LPLAT superfamily.Temporal and spatial expression results showed that the highest expression of BnLPAT5 was in root and the expression levels was two times of stem and embryo.Stress analysis indicated that BnLPAT5 presented two kinds of expression patterns under the treatments of NaCl,PEG4000,waterlogging,6BA and ABA,and which limited to the expression of BnLPAT5.Under the treatment of NaCl,PEG4000 and water logging,BnLPAT5 presented a model of,rapidly fall and then slowly ascent and the minimum was at 3 h after treatment.As for the treatment of 6BA and ABA,BnLPAT5 presented a model of slowly ascent and then slowly fall' and the minimum was at 12 h after treatment.Compaired the influence of,high-salt,arid,water logging,6BA and ABA,range analysis showed those factors regulated the expression of BnLPAT5 in the similarity level.In comparison,high-salt's inflluence was more obvious.A copy of LPAT5 was isolated from Brassica napus and analysed its temporal and spatial expression and stress expression for the first time.We thought BnLPAT5 was a ubiquitous expression gene and sensitive to the arid.

Key words: LPAT5, Brassica napus, Temporal and spatial expression, Abiotic stress, Expression analysis

CLC Number:

XIAO Dan-wang, LIU Cong, CHEN She-yuan, GUAN Chun-yun, XIONG Xing-hua. Cloning and Expression Analysis of LPAT5 from Brassica napus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(5): 33-38. doi: 10.7668/hbnxb.2014.05.007.

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