ACTA AGRICULTURAE BOREALI-SINICA ›› 2014, Vol. 29 ›› Issue (5): 150-155. doi: 10.7668/hbnxb.2014.05.026

Special Issue: Tobacco Biotechnology

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Effects of Temperature Range and Light Intensity on Content of CBT-diols and Expression of CYC-1 and CYP71D16 in Tobacco

WANG Dong1, ZHANG Xiao-quan1, PENG Yu-fu2, ZHANG Di3, LI Li-hua1, YANG Tie-zhao1   

  1. 1. College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, China;
    2. Technology Center of China Tobacco Henan Industrial Corporation, Zhengzhou 450002, China;
    3. Xuchang Cigarette Factory of China Tobacco Henan Industrial Corporation, Xuchang 461000, China
  • Received:2014-07-13 Published:2014-10-28

Abstract: Using 8306 and 12451 tobacco cultivars as the materials,the effects of temperature range between day and night and light intensity on the content of CBT-diols and the expression of CYC-1 and CYP71D16 were studied.When the temperature range was 15 ℃,the content of CBT-diols and the expression levels of CYC-1 and CYP71D16 were significant higher than those in the temperature range of 0 ℃.This suggests that larger temperature range could promote the up-regulated expression of CYC-1 and CYP71D16,so the CBT-diols accumulated more sufficiently.In the condition of 100 000 lx light intensity,the content of CBT-diols and the expression levels of CYC-1 and CYP71D16 were significantly higher than those in the condition of 40 000 lx light intensity.This suggests that shading inhibited the expression of CYC-1 and CYP71D16 and larger light intensity promoted the synthesis of CBT-diols.

Key words: Cembratrien-diol, Gene expression, Temperature range between day and night, Light intensity

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Cite this article

WANG Dong, ZHANG Xiao-quan, PENG Yu-fu, ZHANG Di, LI Li-hua, YANG Tie-zhao. Effects of Temperature Range and Light Intensity on Content of CBT-diols and Expression of CYC-1 and CYP71D16 in Tobacco[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(5): 150-155. doi: 10.7668/hbnxb.2014.05.026.

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