DistRibution of Allelic VaRiation of DwaRfing Genes Rht-B1b and Rht-D1b in Xinjiang SpRing Wheat GeRmplasm ResouRces

doi:10.7668/hbnxb.2013.06.010

Abstract

Abstract: In order to provide crucial information and methods of molecular marker-assisted selection for dwar-fing breeding in Xinjiang spring wheat.A total of 270 spring wheat germplasm resources from Xinjiang wheat re-gions,including 49 landraces, 144 introduced cultivars and 77 local cultivars in Xinjiang, were identified for the al-lelic variation composition of dwarfing gene Rht-B1b(Previously Rht1) and Rht-D1b(Previously Rht2) by using STS markers(NH-BF.2/WR1.2,NH-BF.2/MR1,DF/WR2.4 and DF/MR2) at two loci Rht-B1 and Rht-D1.The results indicated that the percentages of cultivars with gene Rht-B1a(Wildtype),Rht-B1b(Mutation),Rht-D1a (Wildtype),Rht-D1b(Mutation) are 63.3%,36.7%,42.2%,57.8%,respectively.And allelic variation Rht-B1a and Rht-D1 b are main type at Rht-B1 and Rht-D1 loci in Xinjiang spring wheat germplasm resources.the number of cultivars(Lines) with one dwarfing gene(Rht-B1b or Rht-D1b) is 233 and its percentage is 86.3%,the number of cultivars with two dwarfing genes is 11 and its percentage is 4.1%,the number of cultivars neither Rht-B1b gene nor Rht-D1b gene is 26 and its percentage is 9.6%.The percentages of dwarfing gene Rht-B1b and Rht-D1b are ob-viously different in Xinjiang landraces, local cultivars and introduced cultivars, its is 20.4%, 87.8%, 72.7%, 24.7%,22.9%,65.3%,respectively.In summary,cultivars with dwarfing genotype Rht-B1b should be mainly se-lected for the improvement of plant height in Xinjiang spring wheat.The functional markers of two dwarfing gene (Rht-B1b and Rht-D1b) can be directly used for promoting the breeding efficiency.

Key words: Xinjiang, SpRing wheat, GeRmplasm ResouRces, DwaRfing gene, Allelic vaRiation, STS maRkeR

CLC Number:

S512.12

WANG Liang, LI Wei-hua, XU Hong-jun, LU Peng-jun, CUI Feng-juan, LI Shi-lei. DistRibution of Allelic VaRiation of DwaRfing Genes Rht-B1b and Rht-D1b in Xinjiang SpRing Wheat GeRmplasm ResouRces[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(6): 59-64. doi: 10.7668/hbnxb.2013.06.010.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2013.06.010

http://www.hbnxb.net/EN/Y2013/V28/I6/59

References

[1] 何中虎,张爱民.中国小麦育种研究进展[M].北京:中国科学技术出版社, 2002: 369-375.
[2] 张晓科.中国小麦矮秆基因和春化基因分布及小麦品质相关性状多重PCR 体系建立[D].北京: 中国农业科学院, 2007.
[3] 贾继增,丁寿康,李月华,等.中国小麦的主要矮秆基因及矮源的研究[J].中国农业科学, 1992,25( 1): 1-5.
[4] McIntosh R A,Hart G E,Devos K M, et al.Catalogue of gene symbols for wheat[M]//Slinkard A E( ed) Proc 9th Intern Wheat Genet Symp Volume 5, 1998 August 2-7, Saskatoon ( Saskatchewan), Canada: University Extension press,University of Saskatchewan( Saskatoon), 1998: 1 －12.
[5] 赵和.小麦矮秆基因研究和利用现状[J].河北农业科学, 2004,8( 4): 96-99.
[6] Allan R E,Vogel O A,Craddock J C.Comparative response to gibberellic acid of dwarf,semi-dwarf and standard short and tall winter wheat varieties[J].Agronomy Journal,1959,51: 737-739.
[7] Li X P,Liu Y P,Lan S Q, et al.The value of different GA insensitive Rht dwarfing genes in winter wheat breeding[J].Scientia Agriculture Sinica,2004,37 ( 5 ): 392-394.
[8] Ellis M H,Spielmeyer W,Rebetzke G J, et al." Perfect" markers for the Rht-B1b and Rht-D1b dwarfing genes in wheat[J].Theoretical and Applied Genetics,2002,105:1038-1042.
[9] Zhang X K,Yang S J,Zhou Y, et al.Distribution of the Rht-B1b,Rht-D1b and Rht8 reduced height genes in autumn- sown Chinese wheat detected by molecular markers[J].Euphytica,2006,152: 109-116.
[10] 杨松杰,张晓科,何中虎,等.用STS 标记检测矮秆基因Rht-B1b 和Rht-D1b 在中国小麦中的分布[J].中国农业科学, 2006,39( 8): 1680-1688.
[11] 慕美财,刘勇,郭小丽,等.山东小麦品种中矮秆基因Rht-B1b、Rht-D1b 分布的分子鉴定[J].分子植物育种, 2005,3( 4): 473-478.
[12] 马东钦,王晓伟,许兰杰,等.黄淮麦区部分小麦种质资源中矮秆基因的分布[J].河南农业大学学报,2009,43( 2): 118-125.
[13] 魏凌基,张晶,林海荣,等.新疆育成春小麦和推广品种中矮秆基因Rht-B1b、Rht-D1b 分布的分子鉴定[J].种子, 2008,27( 5): 80-82.
[14] 王亮,穆培源,徐红军,等.新疆小麦品种Wx 基因组成及其淀粉糊化特性研究[J].麦类作物学报,2010,30( 6): 1017-1022.
[15] 庄巧生.中国小麦品种改良及系谱分析[M].北京:中国农业出版社, 2003.
[16] 张晶,吕新华,林海荣,等.新疆育成春小麦品种和推广品种对赤霉素反应的研究[J].新疆农业科学,2007,44( 3): 293-297.
[17] 樊哲儒,李剑峰,张跃强,等.新疆春小麦主栽品种产量潜力研究初报[J].新疆农业科学,2008,45 ( 4):579-583.
[18] 张跃强,李剑峰,王钊英,等.新疆主栽和新育成春小麦品种产量潜力及其构成因素对产量的影响[J].新疆农业科学, 2010,47( 7): 1406-1411.

Please choose a citation manager

Content to export