Study on RNA Editing in Chinese Cabbage CMS96 and MaintaineR Line

doi:10.7668/hbnxb.2013.06.008

Abstract

Abstract: RNA editing is an important biological processing of RNA.It is one of reasons of male sterility for making genes abnormal or non-functional.RNA editing of atp6 and coxⅠgenes in male-sterile line CMS96 and it' s maintainer line were analyzed.Results showed that 14 editing sites were observed in atp6 gene of CMS96.Of these, 12 editing sites changed characteristics of amino acid and increased protein hydrophilicity.In atp6 gene of CMS96 line,base substitutions of T→A and G→U occurred at the 5th and 11th editing sites created two stop co-dons TAG and UGA that led to new ORFs.17 editing sites were observed in coxⅠgene of CMS96.Of these,10 edi-ting sites changed characteristics of amino acid and increased protein hydrophobicity.Most editing sites of atp6 and coxⅠgenes happened at 1th and 2th positions of codons.Except for C→U, new types of A→G,G→A,T→G,G→U,T→C,T→A and C→A were found.There were more editing sites in CMS 96 than those of maintainer line.

Key words: Chinese Cabbage, Male steRile CMS96, MaintaineR line, RNA editing

CLC Number:

S634.1

ZHANG De-shuang, LI Zhen-xing, YU Shuan-cang, ZHANG Feng-lan, YU Yang-jun, ZHAO Xiu-yun, WANG Wei-hong. Study on RNA Editing in Chinese Cabbage CMS96 and MaintaineR Line[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(6): 42-52. doi: 10.7668/hbnxb.2013.06.008.

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http://www.hbnxb.net/EN/Y2013/V28/I6/42

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