Molecular Mapping of Leaf Rust Resistance Genes in Wheat Line L224- 3

doi:10.3969/j.issn.1000-7091.2013.03.015

Abstract

Abstract: Wheat line L224-3 showed high resistance to most of Puccinia triticina pathotypes in the seedling and adult stage in the field． Identify and map the leaf rust resistance gene( s) in L224-3 is very useful to breed wheat cultivars with durable resistance to leaf rust． L224-3 and 37 check lines with known leaf rust resistance genes were inoculated with 15 pathotypes at seedling stage to postulate gene in L224-3． F2: 3 population from the cross L224-3 /Zhengzhou 5389 were identified with pathotype FHDQ for genetic analysis of leaf rust resistance in L224-3．SSR and STS markers were used to map the gene in L224-3． L224-3 might contain a dominant resistance gene， temporarily designated LrL224． Totally four SSR markers ( barc8，gwm582，wmc419，wmc694) and one STS marker ( ω-secali /Glu-B3) on 1B were closely linked to LrL224． The two flanking SSR loci were barc8 and gwm582，with the genetic distances of 4． 3 and 4． 6 cM， respectively． LrL224 was located on 1BL，and it showed different seedling reaction with other genes on 1B． Therefore LrL224 is likely to be a new leaf rust resistance gene．

Key words: Wheat, Leaf rust resistance gene, Gene postulation, Genetic analysis, Molecular mapping

CLC Number:

S435.1

Li Huan, Zheng Manman, Wang Cuifen, Fan Linlin, Li Yaning, Li Zaifeng, Liu Daqun. Molecular Mapping of Leaf Rust Resistance Genes in Wheat Line L224- 3[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(3): 77-81. doi: 10.3969/j.issn.1000-7091.2013.03.015.

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