The Gene Cloning and Bioinformatics Analysis of Endo-β-1，4 -glucanas Gene of Chili Pear

doi:10.3969/j.issn.1000-7091.2013.03.010

Abstract

Abstract: One full-length cDNA sequence of homologous gene endo-β-1，4-glucanas from Pyrus bretschneideri Rehd． Cv． Chili pear was cloned by polymerase chain reaction ( PCR) and RACE method，which was named as PbEG． The PbEG was 1 971 bp and the open reading frame encoded 493 amino acids，while the estimated molecular weight and isoelectric point of the putative protein were 54． 596 1 kDa and 9． 14． The protein encoded by PbEG belonged to Glycosyl-hydrolases-family-9． There was no membrane-spanning domain in the protein but a signal peptide in the N-terminus． And the deduced amino acids homology analysis indicated that PbEG has a high homology with RcEG(84%).

Key words: Pear, Endo-β-1, 4-glucanas, Bioinformatics

CLC Number:

Zhang Xiaofei, Zhang Xianan, Wang Ran, Zhang Xinfu, Yang Shaolan. The Gene Cloning and Bioinformatics Analysis of Endo-β-1，4 -glucanas Gene of Chili Pear[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(3): 53-57. doi: 10.3969/j.issn.1000-7091.2013.03.010.

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