Prokaryotic Expression Analysis of Transcription Factor TaSIM from Triticum aestivum L． YU Yue-hua，gENG Hong-wei， CHEN Quan-jia，gAO Wen-wei， WANG

doi:10.3969/j.issn.1000-7091.2013.02.011

Abstract

Abstract: To investigate the function of TaSIMgene,the cDNA of TaSIMgene was amplified by PCR using pJET1. 2-TaSIM as template and the full-length open reading frame was fused into a prokaryotic expression vector pET-28a. PCR and sequencing showed that the recombinant vector pET-28a-TaSIM was successfully constructed and transformed into E. coli BL21(DE3) cells. The results indicated that the pET-28a-TaSIM with the predicted molecular weight of about 33 kDa was successfully expressed in E. coli BL21(DE3) strain. SDS-PAGE indicated that the best expression quantity was induced with 0.5 mmol/L IPTG treatment for 2 h at 37℃.

Key words: Wheat, TaSIM, Prokaryotic expression

CLC Number:

Q785

YU Yue-hua,gENG Hong-wei, CHEN Quan-jia,gAO Wen-wei, WANG Li-ping, QU Yan-ying. Prokaryotic Expression Analysis of Transcription Factor TaSIM from Triticum aestivum L． YU Yue-hua，gENG Hong-wei， CHEN Quan-jia，gAO Wen-wei， WANG[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(2): 60-62. doi: 10.3969/j.issn.1000-7091.2013.02.011.

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