Production of Specific Proteins duringgraft Compatibility/Incompatibility Response of Heterograft Combination of Cucurbitaceae

doi:10.3969/j.issn.1000-7091.2013.02.004

Abstract

Abstract: The production of specific proteins duringgraft compatible/incompatible heterograft combination of cucurbitaceae was studied by SDS-PAGE electrophoresis. The seedlings of watermelon(Citrullus lanatus(Thunb.)) cultivar 8424 and melon(Cucumis melo L.) cultivar Yongtian No. 3 weregrafted onto the compatible rootstock of winter squash(Cucurbita maxima duch. ex lam) A(TLZ-B2-1-24) and incompatible rootstock winter squash B(Z079-1-2-17),to format the compatibility and incompatibility response of heterograft combination of watermelon/ winter squash A and melon/winter squash A,and watermelon/winter squash B and melon/winter squash B,respec-tively. The results showed as the follows:a decrease in a 39 kDa callus protein band at the winter squash A stock of the compatible union was observed during the 10 days aftergrafting,and the culls proteins of scion(watermelon and melon) were still kept. During the incompatible heterograft stage,two specific proteins(36 kDa for watermelon and 28 kDa for melon) were expressed in the scion,and the original protein of winter squash B were declined seriously and a 38 kDa callus protein were decreased. Those results presented here demonstrated the identification of the pro-teins related tograft compatibility/incompatibility response. These findings allow the assumption that molecular change mechanism takes place duringgraft formation in compatible/incompatible combinations for the first time.

CLC Number:

SONG Hui, ZHANG Xiang-qin, YING Quan-sheng, YAN Li-ying, WANG Ying-er, ZHANG Yong-bing, YAN Lei-yan, WANG Yu-hong. Production of Specific Proteins duringgraft Compatibility/Incompatibility Response of Heterograft Combination of Cucurbitaceae[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(2): 20-26. doi: 10.3969/j.issn.1000-7091.2013.02.004.

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