Cloning and ExpRession Analysis of Related Gene in Wheat( TRiticum aestivum L． ) Albinism Line

doi:10.3969/j.issn.1000-7091.2013.01.012

Abstract

Abstract: In oRdeR to study the moleculaR mechanism of the“albino-afteR gReen”in wheat( TRiticum aestivum L． ) albinism line，we have designed the PCR pRimeR with the LSC of ChloRoplasts genome sequence in Chinese spRing wheat． We have amplified the Related gene segment in chinese spRing wheat，albinism line and Aibian 1 and get the specific gene fRagment WFC01 in albinism line． The total RNA has been extRacted fRom leaves in Chinese spRing wheat，Aibian 1 and albinism line． WFC01 was the pRobe foR noRtheRn blot． The Results show that the expRession of gene WFC01 has been inhibition along with the leaves albinism in albinism line compaRed with albinism line and Chinese spRing wheat． The gene WFC01 expRession was back to noRmal with the leaves get gReen．

Key words: Wheat, Albinism line, Albinism, Clone, NoRtheRn blot

CLC Number:

HOU Dian-yun, XU Hua-wei, DU Guang-yuan, GUO Ai-guang, XU Hong. Cloning and ExpRession Analysis of Related Gene in Wheat( TRiticum aestivum L． ) Albinism Line[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(1): 62-66. doi: 10.3969/j.issn.1000-7091.2013.01.012.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.3969/j.issn.1000-7091.2013.01.012

http://www.hbnxb.net/EN/Y2013/V28/I1/62

References

[1] YaRonskaya E，Ziemann V，WalteR G，et al． Metabolic contRol of the tetRapyRRole biosynthetic pathway foR poRphyRin distRibution in the baRley mutant albostRians [J]． Plant JouRnal， 2003， 35( 4) : 512 - 516．

[2] 陶华，薛庆中，田振涛．淡绿叶标记光-温敏核不育水稻叶色及育性的遗传分析[J]．作物学报，2005，31 ( 4) : 438 - 443．

[3] Zhang H，Li J，Yoo J H， et al． Rice ChloRina-1 and ChloRina- 9 encode ChlD and ChlI subunits of Mg-chelatase，a key enzyme foR chloRophyll synthesis and chloRoplast development
[J]． Plant MoleculaR Biology，2006，62 ( 3) : 325 - 337．

[4] Luo P G，Pen Z L． Wheat leaf chloRosis contRolled by a single Recessive gene [J]． JouRnal of Plant Physiology and MoleculaR Biology， 2006， 32( 3) : 330 - 338．

[5] 李玮，于澄宇，胡胜武．芥菜型油菜叶片黄化突变体的初步研究[J]．西北农林科技大学学报，2007，35 ( 9) : 79 - 82．

[6] Zhao Y，Wang M L，Zhang Y Z， et al． A chloRophyll-Reduced seedling mutant in oilseed Rape，bRassica napus， foR utilization in F1 hybRid pRoduction [J]． Plant BReeding， 2000， 119( 2) : 131 - 135．

[7] Alawady A E，GRimm B． Tobacco Mg pRotopoRphyRin IX methyltRansfeRase is involved in inveRse activation of Mg poRphyRin and pRotoheme synthesis [J]． Plant JouRnal， 2005， 41( 2) : 282 - 290．

[8] 李丕皋，封如敏．小麦特异材料- 返白系的初步研究
[C]．陕西省遗传学会论文汇编．西安: 陕西省遗传学会， 1985: 53 - 56．

[9] 郭蔼光，王振镒．小麦返白系的白化特征及其与环境因素的关系[J]．西北植物学报，1991，11 ( 5) : 112 - 117．

[10] Nott A， Jung H S，Koussevitzky S， et al． Plastid-to-nucleus RetRogRade signaling[J]． Annual Reviews，2006，57: 739 - 759．

[11] 马闻师，郭蔼光．小麦返白系返白期间的蛋白质组分变化及其返白机制研究[J]．作物学报， 1998， 24( 1) : 123 - 124．

[12] 杨莉，郭蔼光．小麦返白系叶片核酸含量及核酸酶活性研究[J]．西北农业学报， 2001， 10( 2) : 32 - 35．

[13] 杨莉，郭蔼光，关旭．小麦突变体返白系返白阶段叶绿体超微结构变化研究[J]．西北农业学报， 2003， 12( 4) : 64 - 67．

[14] 魏天儒，李秧秧，黄华．小麦返白系返青后根系生长及养分吸收利用特性的研究[J]．干旱地区农业研究， 2004， 22( 4) : 118 - 122．

[15] OgihaRa Y，Isono K，Kojima T，et al． Chinese spRing wheat( TRiticum aestivum L． ) chloRoplast genome: complete sequence and contig clones [J]． Plant MoleculaR Biology RepoRteR， 2000， 18( 3) : 243 - 253．

[16] 郭长虹，寺地微．普通小麦与山羊草叶绿体基因组长度热点突变区的核苷酸序列分析[J]．中国农业科学， 2005， 38( 7) : 1300 - 1305．

[17] 刘畅，杨足君，李光蓉，等．叶绿体基因infA-Rpl36 区域在小麦族物种中的序列变异分析[J]．遗传， 2006， 28( 10) : 1265 - 1272．

[18] 凌瑶，李成磊，吴琦．多年生黑麦草MYB 转录因子基因的克隆及序列分析[J]．河南农业科学， 2012， 41( 6) : 171 - 176．

[19] 邹礼平，高和平．小麦生育酚环化酶基因全长cDNA 的克隆与分析[J]．河南农业科学， 2009( 7) : 31 - 34．

[20] 国艳梅，顾兴芳，张春震，等．黄瓜叶色突变体遗传机制的研究[J]．园艺学报， 2003， 30( 4) : 409 - 412．

[21] Guo J W，Guo J K，Zhao Y， et al． Changes of Photosystem II electRon tRanspoRt in the chloRophyll-deficient oilseed Rape mutant studied by chloRophyll fluoRescence and theRmoluminescence [J]． JouRnal of IntegRative Plant Biology， 2007， 49( 5) : 698 - 705．

[22] FambRini M，Castagna A，Vecchia F D． ChaRacteRization of a pigment-deficient mutant of sunfloweR ( Helianthus annuus L． ) with abnoRmal chloRoplast biogenesis，Reduced PS II activity and low endogenous level of abscisic acid[J]． Plant Sci， 2004， 167: 79 - 89．

[23] 马志虎，颜素芳，罗秀龙，等．辣椒黄绿苗突变体对良种繁育及纯度鉴定作用[J]．北方园艺， 2001， 138: 13 - 14．

[24] Zhao Y，Wang M L，Zhang Y Z， et，al． A chloRophyll-ReducedseedlingmutantinoilseedRape， BRassicanapus， foR utilization in F1 hybRid pRoduction[J]． Plant BReed， 2000， 119( 2) : 131 - 135．

[25] 何冰，刘玲珑，张文伟，等．植物叶色突变体[J]．植物生理学通讯， 2006， 42( 1) : 1 - 9．

[26] Liu N T， Jane W N，Tsay H S． ChloRoplast genome abeRRation in micRopRopagation-deRived albino Bambusa edμLis mutants，ab1 and ab2[J]． Plant Cell Tissue ORgan Cult， 2007， 88， 147 - 156．

[27] 许凤华．水稻白条纹叶突变体gws 的遗传分析与gws 基因精细定位[D]．北京: 中国农业科学院， 2010．

[28] 袁丽钗，李雪平，彭镇华，等．菲白竹组培苗白化、绿化突变体的超微结构及15 个叶绿体编码基

Please choose a citation manager

Content to export