Association Analysis of Polymorphism in the Bovine Lactoferrin Gene Exon11 with the Susceptibility to Mastitis and Part Milk Trait in Simmental Cattles

doi:10.3969/j.issn.1000-7091.2012.03.042

Abstract

Abstract: To explore the association of polymorphisms of bovine lactoferrin(bLF)gene with the susceptibility to?mastitis and part milk trait，PCR-SSCP and DNA sequencing techniques were used to detect the polymorphisms of?exon11 of 38 case cattle and 118 control cattle. The results analysis showed that，the extremely significant differences in genotypic frequencies and allelic frequencies of exon11 were present in case group and control group(P ＜?0. 01)，AA genotypic frequencies and A allelic frequencies of case group were extremely significant higher than the?control group(P ＜0. 01). Moreover，the SCS of AA genotype individuals were significant higher than AB and BB?genotype(P ＜0. 05)，the milk protein of AA genotype individuals were extremely significant higher than AB genotype(P ＜0. 01)，and significant higher than BB genotype(P ＜0. 05)，the BB genotype individuals were significant?higher than AB genotype(P ＜0. 05). These findings indicated a prospect，the A allelic of bLF-exon11 may have relevant to the susceptibility of mastitis in Simmental cattle.

Key words: Bovine lactoferrin, Somatic cell count, Mastitis, PCR-SSCP

CLC Number:

S823

WANG Guo-fu, WU Hui-guang, SUN Guo-quan, LI Jun-ya, GAO Xue, WANG Jing-shan, WANG Yu-quan, GAO Shu-xin. Association Analysis of Polymorphism in the Bovine Lactoferrin Gene Exon11 with the Susceptibility to Mastitis and Part Milk Trait in Simmental Cattles[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(3): 218-222. doi: 10.3969/j.issn.1000-7091.2012.03.042.

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