Cloning and Sequence Analysis of Three Betaine Aldehyde Dehydrogenase Genes in Tobacco(Nicotiana tabacum )

doi:10.3969/j.issn.1000-7091.2012.03.004

Abstract

Abstract: In silicon cloning，three betaine aldehyde dehydrogenase genes(NtBADH1、NtBADH2 and NtBADH3)were obtained successfully from tobacco cultivar K326 by using the wolfberry BADHgene sequence?(FJ514799)to be probe. Sequence analysis showed that the in silicon cloned cDNAs had a complete open reading?frame ( ORF ) and encoded 504 amino acid residues，respectively，including the conserved decapaptide( VTLELGGKSP ) and cysteine residue(C)in aldehyde dehydrogenase( ALDHs).The three tobacco BADH genes showed high?similarity at the protein level with that of other species. Phylogenetic tree analysis showed that NtBADHI and NtBADH2 had closest relationship with that from Solanum lycopersacum，and NtBADH3 had closest relationship with?that from Gycium barbarum. Those results is of potential importance not only for further study on the nature and funclion of tobacco BADHs but also for the improvement of crops for abiotic stress resistance by genetic engineering.

Key words: Nicotiana ta6acum, Betaine aldehyde dehydrogenase( BADH), Gene cloning, Sequence analysis

CLC Number:

S572
Q785

LIN Shi-feng, REN Xue-liang, WANG Yi, WANG Dong-mao, ZHANG Tuo, WANG Ren-gang. Cloning and Sequence Analysis of Three Betaine Aldehyde Dehydrogenase Genes in Tobacco(Nicotiana tabacum )[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(3): 17-22. doi: 10.3969/j.issn.1000-7091.2012.03.004.

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