In Silico Cloning and Bioinformatics Analysis of GmMDH1 Gene in Soybean( Glycine max. L)

doi:10.3969/j.issn.1000-7091.2012.03.005

Abstract

Abstract: To obtain more plant high phosphorus-efficiency related genes，one plant high P-efficiency gene，?named malate dehydrogenase gene( MDH)，was isolated from soybean by using the in sildco cloning technique. And?the protein of this gene was also predicted and studied through the bioinformatics analysis method. The results?showed that the cDNA length of soybean malate dehydrogenase gene GmMDHI was 1 574 by，which included a?1 230 by open reading frame ( ORF ) and encoded 409 amino acids residue. The encoding protein of GmMDHI possessed NAD binding site，dimerization interface，substrate binding site，MDH_ glyoxysomal_mitochondrial，LDH_?MDH _like super-family domains and was predicted to locate in cell chloroplast.

Key words: High phosphorus-efficiency related genes, Soybean, Malate dehydrogenase, In silico cloning

CLC Number:

S565.1

LI Wen-shuang, LIU Yuan, CHANG Wen-suo, LIU Meng-xing, LI Xi-huan. In Silico Cloning and Bioinformatics Analysis of GmMDH1 Gene in Soybean( Glycine max. L)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(3): 23-28. doi: 10.3969/j.issn.1000-7091.2012.03.005.

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