Association of INHA and INHBA Genes Polymorphism with Prolificacy of Bamei Mutton Sheep

doi:10.3969/j.issn.1000-7091.2012.03.022

Abstract

Abstract: Single nucleotide polymorphism of the α-inhibin (INHA) and βA-inhibin(INHBA) genes were detected in Bamei mutton sheep by PCR-SSCP. The results indicated that there were polymorphism in the amplified region for three primer pairs (1，4 and 6)，no polymorphism in the amplified region for other three primer pairs. For?primer 1，two genotypes (AA and AB) were detected in Bamei mutton sheep. The ewes with mutation heterozygous?genotype AB had 0. 17 (P ＜0. 01) lambs more than those with Mutation homozygous genotype AA in Bamei mutton?sheep. For primer 4，three genotypes (AA，AB and BB) were detected in Bamei mutton sheep. Sequencing revealed?a mutation(G→A )at 857 bp of Exon 2 of the INHBA gene in genotype DD compared with genotype CC. This mutation did not result amino acid change. The ewes with mutation heterozygous genotype DD had 0. 44 (P ＜ 0. 01)?lambs more than those with Mutation homozygous genotype CC. For primer 6，three genotypes (EE，FF and EF)?were detected. Sequencing revealed a mutation(A→T)at 288 bp of Exon 1 of the INHA gene in genotype FF compared with genotype EE. This mutation resulted amino acid change of Met→Lys. The ewes with mutation heterozygous genotype EE had 0. 21 (P ＜0. 01) lambs more than those with Mutation homozygous genotype FF. These results preliminarily show that the INHA and INHBA genes were important genes that influence the prolificacy in Bamei mutton sheep.

Key words: Bamei mutton sheep, Inhibinα gene, Inhibinβ_A gene, PCR-SSCP

CLC Number:

S826

SUO Feng, LIU Yong-bin, TE Rigele, QI Yun-xia, HE Xiao-long, HAN You-sheng, ZHANG Li, LI Hu-shan, RONG Wei-heng. Association of _INHA and _INHBA Genes Polymorphism with Prolificacy of Bamei Mutton Sheep[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(3): 115-119. doi: 10.3969/j.issn.1000-7091.2012.03.022.

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