Studies on the Nucleic Acid Strandedness and Polarity of Porcine Circovirus-like Agent P1

doi:10.3969/j.issn.1000-7091.2012.03.023

ACTA AGRICULTURAE BOREALI-SINICA ›› 2012, Vol. 27 ›› Issue (3): 120-122. doi: 10.3969/j.issn.1000-7091.2012.03.023

Studies on the Nucleic Acid Strandedness and Polarity of Porcine Circovirus-like Agent P1

WEN Li-bin, HE Kong-wang, NI Yan-xiu, ZHANG Xue-han, LI Bin, WANG Xiao-min, GUO Rong-li, ZHOU Jun-ming, YU Zheng-yu, MAO Ai-hua, LU Li-xin

Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014, China

Received:2012-03-26 Published:2012-06-28

Abstract

Abstract: The results of the nucleic acid strandedness and polarity of porcine circovirus-like agent P1 were reported here. Relatively pure viral particles were obtained by CsCl equilibrium density-gradient centrifugation. The?P1 could be determined as single strand nucleic acid and negative-strand genome by S1 nuclease treatment and two?round of PCR amplification，the first round using the single-stranded-specific primer and the second round by one?pair of specific primers.

Key words: Porcine circovirus-like agent P1, Nucleic acid strandedness, Polarity

CLC Number:

WEN Li-bin, HE Kong-wang, NI Yan-xiu, ZHANG Xue-han, LI Bin, WANG Xiao-min, GUO Rong-li, ZHOU Jun-ming, YU Zheng-yu, MAO Ai-hua, LU Li-xin. Studies on the Nucleic Acid Strandedness and Polarity of Porcine Circovirus-like Agent P1[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(3): 120-122. doi: 10.3969/j.issn.1000-7091.2012.03.023.

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http://www.hbnxb.net/EN/Y2012/V27/I3/120

References

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