Expression of Cysticercus cellulosae M13h Protein and Optimization of ELISA

doi:10.7668/hbnxb.2011.04.012

Abstract

Abstract: An indirect-ELISA was developed based on a recombinant protein M13h of Cysticercus cellulosae.The condition was selected successfully by phalanx titration.The ELISA used an optimal amount of the M13h protein at 1 μg/mL and a serum dilution of 1∶ 100,followed by adding rabbit anti-pig IgG HRP conjugate at 1∶ 1 000 and all incubated for 45 min.The recombinant antigen was highly specific and had no cross reactions with positive serum of toxoplasmosis,trichinelliasis,echinococciasis,ascariasis,cysticercus tenuicollis.Then it had a good repeatability.This indirect ELISA assay would provide a simple and rapid serodiagnosis means for epidemiological survey of cysticercosis.

Key words: Cysticercus cellulosae, M13h protein, Indirect-ELISA

CLC Number:

WANG Qiu-xia, MA Jing-zhou, NING Chang-shen, ZHANG Long-xian, JIAN Fu-chun. Expression of Cysticercus cellulosae M13h Protein and Optimization of ELISA[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(4): 72-76. doi: 10.7668/hbnxb.2011.04.012.

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