Analysis of SSR Information in EST Resource of Tomato (Solanum lycopersicum) Fruit

doi:10.7668/hbnxb.2011.04.038

Abstract

Abstract: Expressed sequence tags(ESTs)are important resources for development of new SSR markers.83 613 ESTs of the tomato fruit in the database of NCBl were downloaded and early treatmented,such as being clusterd to redundancy and spliced,and so on.It is concluted that there were 18 878 UniGene after being spliced by CAP3 tool,in which of 9 650 Contigs and 9 228 Singlets.2 039 EST-SSRs were gotten by using MISA tool searched SSR,accounting for 12.62% of the UniGene and including 97 repeat motifs.Mononucleotide,Dinucleotide and Trinuclotide were the dominant repeat motifs and occupied 98.91% in all the number of SSR.Every 5 451.6 mer appeared a SSR in the ESTs of the tomato fruit of containing SSR.

Key words: Tomato, EST-SSR, Fruit

CLC Number:

HAN Ming-li, CUI Na, YU Zhi-hai, LI Tian-lai, HOU Li-xia. Analysis of SSR Information in EST Resource of Tomato (Solanum lycopersicum) Fruit[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(4): 213-217. doi: 10.7668/hbnxb.2011.04.038.

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