Analysis of EcoR I-O Fragment from Orgyia ericae Nucleopolyhedrovirus

doi:10.7668/hbnxb.2010.01.011

Abstract

Abstract: It is not specified of the molecular characterization on Orgyia ericae Nucleopolyhedrovirus(OrerNPV).Here,the cloned OrerNPV EcoR I-O fragment was analyzed,which the egt gene and homologues of Ol-129 gene were found in this fragment.The OrerNPV egt open reading frame was 1 539 nucleotides long that coded a peptide of 512 amino acids with a predicted molecular weight of 57.9 kDa.The peptide of OrerNPV egt shared a highest similarity of 89% to Orgyia leucostigma nucleopolyhedrovirus;while it shared the lowest similarity with Adoxophyes orana nucleopolyhedrovirus 46%.The homologues of Ol-129 gene amino acid residues in the M13+ terminal of OrerNPV had 89%-40% identities with homologous genes from other 10 NPVs.The result showed that characteristic of OrerNPV EcoR I-O fragment and OrerNPV egt gene.

Key words: Orgyia ericae nucleopolyhedrovirus, EcoR I-O fragment, egt gene, Homologues of Ol-129 gene

CLC Number:

S432.1

YANG Li-rong1,YAN Hai-xia1,QUAN Xin1,XUE Bao-guo1,Dalangbayaer2. Analysis of EcoR I-O Fragment from Orgyia ericae Nucleopolyhedrovirus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(1): 55-60. doi: 10.7668/hbnxb.2010.01.011.

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