Bioinformatics and Expression Analysis of Glutathione S-transferase Gene in Rhizoctonia solani AG1-IA

doi:10.7668/hbnxb.2018.01.008

Abstract

Abstract: The aim of this study is to provide the basic for the exploration of Rsgst genes by bioinformatics and gene expression analysis.Based on the R.solani AG1-IA genome database RSIADB,the sequence of Rsgst gene was found.Basic information of the GST amino acid sequence was predicted by several softwares,and a phylogenetic tree was constructed using MEGA 5.0 software.qRT-PCR was used to detect the gene expression levels during the progress of sclerotial development of R.solani AG1-IA,and the GST activity was measured simultaneously.The results showed that the full length of Rsgst was 1 207 bp,and the gene encodes 277 amino acid residues.The molecular weight of GST was 30.90 ku and the theoretical isoelectric point was 9.42.The secondary structure of this protein was mainly consisted by α-helix which accounted for 41.52%.The phylogenetic tree showed that the R.solani GST was more closely related to Sporisorium reilianum GST.qRT-PCR indicated that the expression of Rsgst was upregulated during the sclerotial development with the highest gene expression at 60 h,which was 7.64,and the maximal activity of GST was 0.375 U/μg at the 5th day.These results are significant to decipher the functions of Rsgst in R.solani AG1-IA.

Key words: Rhizoctonia solani, Glutathione S-transferase, Sclerotial development, Enzyme activity determination, Genomic location

CLC Number:

S432.1
Q78

ZHANG Yunhuan, ZHENG Wenbo, ZHAO Mei, WANG Chenjiaozi, ZHOU Erxun, SHU Canwei. Bioinformatics and Expression Analysis of Glutathione S-transferase Gene in Rhizoctonia solani AG1-IA[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(1): 45-51. doi: 10.7668/hbnxb.2018.01.008.

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