Abstract: In order to study the function and action of the tapetum developmental regulatory gene Udt1 in maize, bioinformatics analysis was carried out in this experiment.By using Udt1 gene to cultivate new male sterile maize varieties,to improve the purity of hybrids and the quality of germplasm and shorten the breeding years.The study used the known rice OsUdt1 gene (AY953870.1) as the reference sequence,using homologous cloning,the highest homology gene BT068494.1(GenBank) was obtained in maize genome,and bioinformatics was used to predict the structure and biological function of the gene.At the same time,the molecular phylogenetic tree was constructed by using MEGA 7.0 software.The ZmUdt1 gene was located on second chromosomes of maize,ZmUdt1 protein was composed of 219 amino acids.The relative molecular weight of the protein was 24.48 ku and the isoelectric point was 5.35,belonging to hydrophilic protein.It was mainly composed of alpha helix and regular coil,forming two structures.The three stage structure was basically consistent with the three stage structure of OsUdt1 protein in rice,belong to the HLH super family.There were no signal peptides and transmembrane structures; Subcellular localization predicts that the protein had more likely to be located in the nucleus.It was predicted that the protein had functions of translation,replication and transcription,transcriptional regulation and signal transduction,and it had 17 phosphorylation sites,whether it had any other function had not been determined.Phylogenetic tree showed that the genetic distance between maize and rice,sorghum and other species was the closest,and also had a certain genetic correlation with Arabidopsis thaliana DYT1 protein.By comparison and prediction,the structure and function of ZmUdt1 and OsUdt1 proteins were highly similar.This experiment provides a theoretical basis for further research on the function and function of ZmUdt1 protein and the innovation of male sterile lines of maize.

Key words: ZmUdt1 gene, Maize, Bioinformatics analysis

CLC Number: 

• Q78

• S513.03