Analysis of Polymorphisms of bLF exon4 and the Relationship between the Gene and Mastitis in Chinese Simmental Cattles

doi:10.7668/hbnxb.2010.01.010

Abstract

Abstract: This study is to investigate the association between Bovine Lactoferrin gene exon4 polymorphism and mastitis.In this experiment,the genotype distribution of polymorphism fragment in bLF gene exon4 was analyzed by PCR-SSCP in 86 Chinese Simmental cattles.Meanwhile,the effect of bLF gene exon4 on mastitis was analyzed.It showed that:the distribution of three genotypes was significantly different(P<0.05) of healthy dairy cows by using x² analysis.The BB genotype frequency was higher in the bovine mastitis cases,which suggested that this polymorphism could be related to bovine mastitis affection and might become a molecularmarker of the mastitis.

Key words: Chinese Simmental Cattles, Bovine lactoferrin, Mastitis, Somatic cell count, PCR-SSCP

CLC Number:

WANG Guo-fu1,WU Hui-guang2,SUN Guo-quan3,XU Shang-zhong4,LI Jun-ya4,GAO Xue4,JI Shou-cai5,GAO Shu-xin1. Analysis of Polymorphisms of bLF exon4 and the Relationship between the Gene and Mastitis in Chinese Simmental Cattles[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(1): 50-54. doi: 10.7668/hbnxb.2010.01.010.

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