ACTA AGRICULTURAE BOREALI-SINICA ›› 2010, Vol. 25 ›› Issue (1): 217-221. doi: 10.7668/hbnxb.2010.01.044

Special Issue: Wheat Biotechnology

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Physiological Indicators of Near-isogenic Wheat Lines in Interaction with Powdery Mildew

GUAN Xi-zhen,ZHANG Wei-dong,TIAN Ji-chun   

  1. Key Laboratory of Crop Biology of Shandong Province, Group of Quality Wheat Breeding, Shandong Agricultural University, Tai′an 271018, China
  • Received:2009-11-03 Published:2010-02-28

Abstract: Bainong3217 is a sensitive wheat variety to powdery mildew,and one of its near isogenic lines,Xbd/Bainong3217(BC7F6),includes a disease resistant gene xbd and could resist to powdery mildew.The two lines were used as materials in this experiment.After inoculated by Blumeria graminis,E09,the wheat leaves were detected on concentrations of hydrogen peroxideanalysis(H2O2)and biological activities of seven kinds of metabolic enzymes during 0-6 days.These enzymes include superoxide dismutase(SOD),catalase(CAT),glutathione reductase(GR),glutathione peroxidase(GPX),dehydroascorbate reductase(DHAR),ascorbate peroxidase(APX)and glutathione S-transferase(GST).The aim of the study is to evaluate differences of physiological indicators between the two susceptible and resistant wheat lines during the pathogen infection period.The results showed that the H2O2 content in the susceptible and resistant strains were significantly decreased and increased after inoculation with powdery mildew,respectively; CAT activity was not significantly different in the susceptible or resistant strains during infection; SOD,GR,GPX,APX,and GST activities in the leaves of susceptible line increased significantly during post-inoculation,and were higher than those in the resistant lines; However,DHAR activity changed with no obvious trend.During wheat powdery mildew infection,H2O2 might play an important role in the wheat responses.Several metabolic mechanisms might also involve in the responses,and H2O2 content could be influenced by these various enzymes.

Key words: Wheat, Powdery mildew, Interaction, Physiological indicators

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Cite this article

GUAN Xi-zhen,ZHANG Wei-dong,TIAN Ji-chun. Physiological Indicators of Near-isogenic Wheat Lines in Interaction with Powdery Mildew[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(1): 217-221. doi: 10.7668/hbnxb.2010.01.044.

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