Gene Cloning and Sequence Analysis of Fatty Acid Elongase 1(fae1) in Sinapis arvensis L.

doi:10.7668/hbnxb.2009.05.009

Abstract

Abstract: PCR primers were designed according to the fae1 (Fatty acid elongase 1.fae1) DNA sequence of Brassica napus Zhongshuang 9 reported in GenBank (Accession No.AY888037),PCR was carried out with genomic DNA of Sinapis arvensis as template and the specific fragment was purified and cloned.Result of sequencing showed that the fragment was 1651 bp and analysis of sequence indicated that the coding region was 1521 bp and encoded 506 amino acids.Results of BLASTn demonstrated that the fae1 from Sinapis arvensis has 76%-98% nucleotide similarity with genes cloned from Brassica species,and BLASTp demonstrated that 32 amino acids residue substitutions were found between Sinapis arvensis and <>iBrassica species,some sequence changes could explain lines with different erucic acid content.

Key words: Sinapis arvensis L., Erucic acid, Fatty acid elongase 1(fae1), Gene cloning, Sequence analysis

CLC Number:

Q785

ZHAO Fu-yong, GAO Zhen, YAN Han, TIAN Zhi-hong. Gene Cloning and Sequence Analysis of Fatty Acid Elongase 1(fae1) in Sinapis arvensis L.[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(5): 40-44. doi: 10.7668/hbnxb.2009.05.009.

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