The Physiological,Biochemical and Chloroplast Proteomic Changes of Wheat Under the Induction of Copper

doi:10.7668/hbnxb.2009.05.029

Abstract

Abstract: The aim of studying the effects of heavy metal copper which is one of the heavy metal pollutants on the physiological,biochemical characteristics and chloroplast proteome of the wheat seedlings is to explore its influence on the growth of wheat seedlings and chloroplast proteome.Treat the wheat seedlings with CuCl₂ for 5 days,measure the length of seedlings and determine the content of chlorophyll and soluble protein,the results indicated that with the concentration of Cu²⁺ increased,the height of wheat seedlings significantly decreased,the height of seedlings under each concentration was significantly lower than the control,54.47% decreased with the concentration of 5 mmol/L in contrast to the control;the chlorophyll content showed the trend of decrease-increase-decrease,but all less than the control;the soluble protein content decreased 0.68-1.23 mg/g in contrast to the control with no significant distinction.Analyze the change of chloroplast proteome by two-dimensional electrophoresis (2-DE) with the Cu²⁺ concentration of 1 mmol/L,the result showed that 17 protein spots significantly changed,seven spots disappeared,one spot appeared,and nine spots down-expressed.

Key words: Copper, Wheat, Chlorophyll, Soluble protein, 2D-PAGE

CLC Number:

FAN Bao-li. The Physiological,Biochemical and Chloroplast Proteomic Changes of Wheat Under the Induction of Copper[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(5): 140-143. doi: 10.7668/hbnxb.2009.05.029.

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http://www.hbnxb.net/EN/Y2009/V24/I5/140

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