Cloning and Functional Characterization of a Saccharomyces cerevisiae Hog1 MAPK Homologous Gene AtHOG1 from the Fungus Alternaria tenuissima

doi:10.7668/hbnxb.2009.04.015

Abstract

Abstract: An Alternaria tenuissima cDNA expression library was constructed. The MAP kinase HOG1 was isolated from the Alternaria tenuissima cDNA expression library,designated as AtHOG1. It had a size of 1 539 bases in length,en2 coded a protein of 355 amino acids. The AtHog1p contained the conserved TGYactivation loop found in the stress2activat2 ed protein kinase subgroup of MAPKs and its amino acid sequence showed 94 %,90 % and 80 % identities with AfOsm1p (XP2752664)of Aspergillus f umigatus,Mg01822 (XP2363896)of Magnaporthe grisea and ScHog1p (AAB67558)of Sac2 charomyces cerevisiae, respectively. AtHOG1 cDNA sequences could complement the functions of S. cerevisiae ScHOG1 genes in sodium chloride tolerance,suggesting a functional HOGpathway exists in A. tenuissima,AtHOG1 gene was in2 volved in the stress adaptation regulation of A. tenuissima.

Key words: :High osmolarity glycerol MAP kinase, Alternaria tenuissima, HOG1, Stress adaptation

CLC Number:

Q785

FENG Fei, JI Chun-yan, YANG Xiu-fen, ZENG Hong-mei, QIU De-wen. Cloning and Functional Characterization of a Saccharomyces cerevisiae Hog1 MAPK Homologous Gene AtHOG1 from the Fungus Alternaria tenuissima[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(4): 74-79. doi: 10.7668/hbnxb.2009.04.015.

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