The Cloning and Analysis of NDK-1 cDNA Derived from Rainbow Trout (Oncorhynchus mykiss)

doi:10.7668/hbnxb.2009.04.014

Abstract

Abstract: RT-PCR and RACE (Rapid Amplification of cDNA Ends)methods were used for the isolation of the fulllength cDNA of NDK-1 gene (GenBank accession number :FJ591154)from rainbow trout.Sequence analysis revealed a 953 bp cDNA containing the 45 bp 5′-UTR, 284 bp 3′-UTR and 624 bp ORF encoding 207 amino acids.Sequence alignment of NDPK amino acid between rainbow trout and salmon, zebrafish,western frog, africa frog, human and fugu exhibited 95 %, 72 %, 68 %, 68 %, 65 % and 64 %identity rate, respectively.The result indicated the NDK-1 gene was highly conservative in the progress of evolution.

Key words: words:Oncorhynchus mykiss, NDK-1 gene, RT-PCR, RACE

CLC Number:

S917

WANG Jia-qing, LIU Xiao-hui, LI Dai-zong, HAN Jin-cheng, GUO Ran, WANG Yue-hong, WANG Zhi-ping,. The Cloning and Analysis of NDK-1 cDNA Derived from Rainbow Trout (Oncorhynchus mykiss)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(4): 70-73. doi: 10.7668/hbnxb.2009.04.014.

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