The Cloning and Analysis of NDK-1 cDNA Derived from Rainbow Trout (Oncorhynchus mykiss)

doi:10.7668/hbnxb.2009.04.014

Abstract

Abstract: RT-PCR and RACE (Rapid Amplification of cDNA Ends)methods were used for the isolation of the fulllength cDNA of NDK-1 gene (GenBank accession number :FJ591154)from rainbow trout.Sequence analysis revealed a 953 bp cDNA containing the 45 bp 5′-UTR, 284 bp 3′-UTR and 624 bp ORF encoding 207 amino acids.Sequence alignment of NDPK amino acid between rainbow trout and salmon, zebrafish,western frog, africa frog, human and fugu exhibited 95 %, 72 %, 68 %, 68 %, 65 % and 64 %identity rate, respectively.The result indicated the NDK-1 gene was highly conservative in the progress of evolution.

Key words: words:Oncorhynchus mykiss, NDK-1 gene, RT-PCR, RACE

CLC Number:

S917

WANG Jia-qing, LIU Xiao-hui, LI Dai-zong, HAN Jin-cheng, GUO Ran, WANG Yue-hong, WANG Zhi-ping,. The Cloning and Analysis of NDK-1 cDNA Derived from Rainbow Trout (Oncorhynchus mykiss)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(4): 70-73. doi: 10.7668/hbnxb.2009.04.014.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2009.04.014

http://www.hbnxb.net/EN/Y2009/V24/I4/70

[1]	LI Ziyu, YAO Yurong, HAO Yongjuan, HUO Jianfei, BEN Haiyan, WANG Xudong, WANG Wanli, YAO Xingwei, LI Erfeng. Transcriptome Analysis and Resistance Gene Mining of Resistant and Susceptible Cauliflower Cultivars in Response to Black Rot Infection [J]. Acta Agriculturae Boreali-Sinica, 2025, 40(2): 161-172.
[2]	WANG Xiaoyue, CHEN Shuangshuang, QI Xiangyu, FENG Jing, CHEN Huijie, SUN Ming, DENG Yanming. Bioinformatics Analysis and Expression Profiling of the Aluminum Transporter HmALMT11 in Hydrangea macrophylla [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(4): 94-101.
[3]	HU Xinyue, WANG Jin, NIE Wanhong, GONG Chao, XU Benbo, ZHANG Xuekun, ZHAO Fuyong. Expression Pattern of FAE1s and Erucic Acid Accumulation in Developing Seeds of Crambe abyssinica [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(3): 35-42.
[4]	WU Yinyin, WEN Siyi, HAN Zhuoran, SUN Jingfeng. Cloning,Tissue Distribution and Temporal Expression Analysis of Synaptosome-associated Protein 29 Gene(SNAP29) in Cynoglossus semilaevis [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(2): 231-238.
[5]	HE Caixia, LI Changzhong, BAO Changhong, WANG Linan, YAN Qingchun, JIN Wenjie, ZHAO Juan, WANG Guojie, JIAN Shenglong, WANG Zhenji, CHEN Yanxia. Cloning and Expression Analysis of MSTN-1 Gene in Gymnocypris eckloni [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(1): 228-238.
[6]	SONG Yunjing, TIAN Yanmei, MENG Ke, YOU Kemei, FENG Dengzhen. Screening and Analysis of LncRNA Related to Muscle Development in Longissimus Dorsi Muscle of Different Sex Tan Sheep [J]. Acta Agriculturae Boreali-Sinica, 2024, 39(1): 219-227.
[7]	HUANG Miao, LUO Youyou, NI Rui, ZHAO Jin. *Cloning and Expression Analysis of ARP* Gene in Capsicum annuum L.var.fasciculatum** [J]. Acta Agriculturae Boreali-Sinica, 2023, 38(5): 69-76.
[8]	LIU Fang, QU Shuo, SUN Haowen, JIANG Haipeng, HAN Yingpeng. Identification and Bioinformatical Analysis of GRAS Gene Family in Response to Soybean Cyst Nematode [J]. Acta Agriculturae Boreali-Sinica, 2023, 38(5): 166-178.
[9]	LIU Xiaolong, LIAO Jingpeng, ZHONG Xin, DUAN Ximiao, HU Yongxuan, LIU Jiacheng, LIU Zekai, YANG Hongtao. Expression Pattern of the OsRboh Family Genes in Rice under Heat Stress of Different Growth Stages [J]. Acta Agriculturae Boreali-Sinica, 2023, 38(5): 1-9.
[10]	WANG Kexin, GUO Zhaoyang, YIN Yuhang, CHEN Shengzhong, SONG Xiyun, ZHAO Meiai. *Cloning and Expression Characteristics of ZmGST* Gene in Maize** [J]. Acta Agriculturae Boreali-Sinica, 2023, 38(4): 20-27.
[11]	WANG Peng, TIAN Zhejuan, ZHAO Xuefang, KANG Chen, WU Zhiming, LI Yadong, HUANG Jinan. *Cloning and Expression of SlCaM3,SlCaM4* and SlCaM5 Genes under Cold Stress in Tomato** [J]. Acta Agriculturae Boreali-Sinica, 2023, 38(2): 75-84.
[12]	XIAO Hong, HE Zhen, TIAN Qi, HUANG Xinmiao, LIAO Weidong, HUANG Tinghua, YAO Min. *Construction of a Fluorescent-labeled Localization Reporter Strain of Salmonella typhimurium* and Its Tracer Application in Porcine Alveolar Macrophages** [J]. Acta Agriculturae Boreali-Sinica, 2023, 38(2): 232-238.
[13]	SHI Yumei, CHEN Shaokang, XING Kai, ZHAO Yanhui, YUAN Jiani, SHENG Xihui, QI Xiaolong, NI Hemin, GUO Yong, WANG Chuduan. Identification of Differential Genes of Longissimus Dorsi Muscle of Songliao Black Pig and Landrace Pig Based on High-throughput Sequencing Technology [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 222-229.
[14]	LIANG Peng, ZHANG Wen, FENG Dengzhen, QIANG Hao, RONG Xuan, MENG Ke. Screening Candidate Genes Related to Meat Quality Traits in Sheep Based on Transcriptome [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(4): 220-231.
[15]	FENG Liting, ZHANG Jianfeng, CHI Shengqi. Establishment of a Multiplex RT-PCR Assay for Detection of Four Sweet Potato RNA Viruses [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(4): 206-211.

Please choose a citation manager

Content to export