Abstract: The genetic ploymorphisms of 297 Chinese Holstein cows with family trees and production rEcords were investigated by using PCR-RFLP and CRS RFLP.Three mutational polymorphic sites,10098(T/G),10111.(G/A)and 11541(C/T),were found in theTLR2gene exon 2 The single nucleotide change at posit ion 10098 and 10111.sit es resulted Asp63Glu and Gly68Ser respectively.In the 10098 site there were three gene types AA,AB and BB,theallelic frequencies of A and B were 0.7845 and 0.2155 respectively.In the 10111.site there were two gene types CC and CD,the allelic frequencies of C and D were 0.9858 and 0.0142 respectively.In the 11541.site there were three gene types EE,EF and FF,the allelic frequencies of E and F were 0.0993 and 0.1434 respectively.Those three polymorphic sites in theTLR2gene exon 2 were in accordance with the Hardy-Weinberg equilibrium(p>0.05).In the 10098 and 10111.and 11541.sites,the heterozygosity were 0.3381,0.0279 and 0.1789 respectively,the number of effective alleles were 1.5108,1.0287 and 1.2179 respectively,the polymorphic information contents were 0.280.9,0.0279 and 0.1434 respectively.The 10098 site was medium polymorphic and the other two sites were low polymorphic.

Key words: Chinese Holstein, TLR2gene, Genetic polymorphisms, PCR-RFLP, CRS-RFLP

CLC Number: 

• Q953+.5