Identification of thevip1A/vip2A Gene from Bacillus thuringiensis Isolates and the Linkage withcry1Gene

doi:10.7668/hbnxb.2008.05.029

Abstract

Abstract: A PCR method and a PCR-restriction　fragment length polymorphism method were　used for identification ofvip1A/vip2A genes andcry1-type genes　from Bacillus thuringiensis isolates,respectively.The result showed thatvip1A/vip2A-type genes appeared in 20　of 117 B.thuringiensis isolates.Avip1A/vip2A gene fragment was cloned　and sequenced.GenBank accession number　is EU594327 Thecry1-type genes were　ident ified ascry1Fa.The results　indicated　that there was some linkage between thevip1A/vip2A andcry1Fa.

Key words: Binary toxin, vip1A /vip2A, vip1A /vip2A-16, PCR-RFLP, cry1Fa

CLC Number:

S476.+1

SHEN Jian-ru, GUO Wei, ZHANG Xia, LI Jing. Identification of thevip1A/vip2A Gene from Bacillus thuringiensis Isolates and the Linkage withcry1Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(5): 131-135. doi: 10.7668/hbnxb.2008.05.029.

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