Zwittermicin A Enhances the Toxicity of Bt Cry1Ac to Spodoptera exigua Hübner

doi:10.7668/hbnxb.2008.05.030

Abstract

Abstract: Zwittermicin A(ZwA)enhanced the insecticidal activity ofBacillus thuringienesis(Bt)Cry1Ac protein.The mortality was 90% at 48 h after S.exigua larvae were treated with Cry1Ac in the presence of ZwA,which was higher than that without ZwA.All the 5th-instar larvae were dead at 84 h after fed with 5 g of Cry1Ac plus 25 g of ZwA(per larvae).Microscopical observation of dissected midgut showed that at 12 h post feeding with Cry1Ac and ZwA no PM were present in the treated larvae,in contrast to the presence of a fully formed PM in the midgut of control larvae.But after 36h,the midgut displayed normal and PM formed again.

Key words: Zwittermicin A, Cry1Ac, Bioassay, Midgut, Peritrophic membrane

CLC Number:

S476+.1

LI Jing, LIU Jian-mi, GUO Wei, ZHANG Xia, SHEN Jian-ru. Zwittermicin A Enhances the Toxicity of Bt Cry1Ac to Spodoptera exigua Hübner[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(5): 136-141. doi: 10.7668/hbnxb.2008.05.030.

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References

[1] Gill S S,Cowles E A,Pietrantonio P V.The mode of action of Bacillus thuringiensis endotoxins[J].Annu Rev Entomol,1992,37:615-636.
[2] 祁红兵,刘铭.苏云金芽孢杆菌的杀虫毒素及其增效方略[J].信阳师范学院学报,2003,1(4):485-491.
[3] Silo-Suh L A,Lethbridge B J,Raffel S J,et al.Biological activities of two fungistatic antibiotic produced by Bacillus cereus UW85[J].Applied and Environmental Microbiology,1994,60:2023-2030.
[4] Shang H Z,Chen J J,Handelsman J,et al.Behavior of Pythium torulosum zoospores during their interaction with tobacco roots and Bacillus cereus[J].Current Microbiology,1999,38:199-204.
[5] Broderick N A,Goodman R M,Raffa K F,et al.Synergy between Zwittermicin A and Bacillus thuringiensis subsp.kurstaki against gypsp moth(Lepidopteria:Lymantriidae)[J].Biological Control,1999,29(1):101-107.
[6] 刘向阳,朱福兴,张凯.甜菜夜蛾抗药性研究现状[J].昆虫知识,2007(5):632-636.
[7] 王瑞明,徐文华,林付根,等.江苏沿海农区甜菜夜蛾发生特点研究进展[J].华东昆虫学报,2007(2):81-86.
[8] Nickerson K W,Julian St G,Bulla L A.Physiology of sporeforming bacteria associated with insects:radiorespirometric survey of carbohydrate metabolism in the 12 serotypes of Bacillus thuringiensis[J].Appl Microbiol,1974,28:129-132.
[9] 郭巍.粉纹夜蛾对苏云金杆菌Cry1Ac的抗性及其防御体系的研究[D].广州:中山大学,2005.
[10] Klein M.An improved per oral administration technique for bioassay of nucleopolyhedrosis viruses against Egyptian cotton worm Spodoptera littoralis.[J].Invertebr Pathol,1978,31:134-136.
[11] 汪世泽.昆虫研究法[M].北京:北京农业出版社,1998:23-33.
[12] 曾晓慧,喻子牛,胡萃.苏云金芽胞杆菌在防治夜蛾科害虫中的应用[J].昆虫天敌,1999,1:38-42.
[13] 王琛柱,钦俊德.棉铃虫幼虫中肠主要蛋白酶活性的鉴定[J].昆虫学报,1996,39(1):7-14.
[14] Nichole A Broderick,Kenneth F Raffa,Jo Handelsman.Midgut bacteria required for Bacillus thuringiensis insecticidal activity[J].Proc Natl Acad Sci USA,2006,103(41):15196-15199.
[15] Ge A Z,Shivarova N I,Dean D H.Location of the Bombyx mori specificity domain on a Bacillus thuringiensis delta-endotoxin protein[J].Proc Natl Acad Sci USA,1989,86(11):4037-4041.
[16] Wang Ping,Robert R.Calcofluor disrupts the midgut defense system in insects[J].Insect Biochemistry and Molecular Biology,2000,30:135-143.

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