Simple Bioassay Method of New Fungicide Against Botrytis cinerea-Cucumber Cotyledon Method

doi:10.3321/j.issn:1000-7091.1991.03.017

Abstract

Abstract: The bioassay model of fungicide characterized by "cucumber cotyledon method" against the grey mold of cucumber caused by Botrytis cinerea has been studied. The repeatability of the experimental result is satisfactory by using this method with the chemicals procymidone and Roval.It has been found that the optimum conidium concentration for inoculation is 1×105 spores/ml, while no disease is produced with the concentration less than 1×102 spores/ml. The age of hypha has significant effect on the result of inoculation, the younger the hypha, the bigger the leaf spot induced.Approximate results have been obtained by using either macerating or spraying method, but the former seems to be even simpler in operation. The surface active agent and solvent used in testing the fungicide has no inhibition effect on B, cinerea except Nong Ru 700 and DMF.

Key words: Gray mold of cucumber, Bioassay, Fungicide

LiShuzheng, ZhangSuhua, LiuShufen, BaoRudi, ZhangRuixian. Simple Bioassay Method of New Fungicide Against Botrytis cinerea-Cucumber Cotyledon Method[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1991, 6(3): 94-99. doi: 10.3321/j.issn:1000-7091.1991.03.017.

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