Clong of Plant Fruit-Specific E8 Promoter

doi:10.7668/hbnxb.2008.03.005

Abstract

Abstract: The DNA was extracted from cotyledons of tomato Maofen 802 by high salt and low pH,step by step acentric,SDS,CTAB and improved CTAB method.The improved CTAB method was the best method in the DNA extract.We use this DNA as stencil and amplified by PCR,the product of which was reclaimed and subcloned into pMD18-T Simple Vector.After identification by PCR and restriction enzymes,the recombinant pMD18-E8 vectors were subjected to sequence analysis.As indicated by homology analysis,the resultant tomato fruit-specific E81.1 promoter was highly conservation,with GenBank submission,proved to share 99.1% homology.Therefore,tomato fruit-specific E8 promoter has been successfully cloned,thus making possible the subsequent research in transgenic peach.

Key words: Tomato, Fruit-specific E8 pmmoter, Gene cloning

CLC Number:

Q785

WANG Yu-xia, LI Wei, WANG Wang-tian, LI Meng-fei, CHEN Xin. Clong of Plant Fruit-Specific E8 Promoter[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(3): 16-19. doi: 10.7668/hbnxb.2008.03.005.

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References

[1] Tong K Z.Gene and Its Expression(基因及其表达)[M].Beijing:Science Press,1998.

[2] 姚嵘,马三梅.植物果实的特异型启动子[J].生命的化学,2006,26(4):336-339.

[3] 张开春,张军科.马哈利樱桃PGIP基因克隆及全序列测定[J].农业生物技术学报,2000,8(3):281-283.

[4] Tai T H,Tanksey D D.Rapid isolation of total DNA from de-hydrated plant tiesue[J].Plant Mol Biol Rep,1990,8(4):229 -303.

[5] Miehaels S D,John M C,Amasino R M.Removal of polysac-charides from plant DNA by ethanol precipitation[J].Biotech-niques,1994,17(2):274-276.

[6] 巩振辉,Cecehimi E,Milner J J.以PCR鉴定转基因植株的微量DNA提取方法[J].西北农业大学学报,1997,25、(1):45-47.

[7] Theresa M F,Seven D T.Mieroprep protocol for extraction of DNA from tomato and other herbaceous plants[J].Plant Molecular Biology Reporter,1995,13(3):207-209.

[8] 顾红雅.植物分子生物学实验手册[M].北京:高等教育出版社,1998:3-12.

[9] 陈绍宁,崔继哲.快速提取番茄总DNA应用于规模化PCR检测[J].北方园艺,2004(3):46-47.

[10] 谭晓风,漆龙霖,黄晓光,等.山茶植物叶片DNA提取[J].中南林学院学报,1999,19(4):78-81.

[11] Deikman J,Fischer R L.Interaction of a DNA binding factor with the 5'-flanking region of an ethylene-responsive fruit ripening gene from tomato[J].EMBOJ,1988,7 (11):3315 -3320.

[12] Deikman J,Kline R,F'iseher R L.Organization of riponing and ethylene regulatory regions in a fruit-speeifie promoter from tomato[J].Plant Physiol,1992,100:2013-2017.

[13] Cordees S,Deikma.n J,Margessian L J,et al.lnteraetian of a develop-mentally regulated DNA-binding factor with sites flanking two different fruit-ripening genes from tomato[J].Plant Cell,1989,1(10):1025-1034.

[14] Coupe S A,Deikman J.Characterization of a DNA-binding protein that interacts with 5'flanking regions of two fruit-ripening genes[J].Plant J,1997,11(6):1207-1218.

[15] 周晓红,陈晓光,张晓东,等.番茄果实特异性E8启动子的基因克隆与序列分析[J].第一军医大学学报,2003,23(1):25-28.

[16] Good X,Kellogg J A,Wagoner W,et al.Reduced ethylene synthesis by transgenie tomatoes expressing S-adenosylme-thionine hydrolase[J].Plant Mol Biol,1994,26 (3):781-790.

[17] Krasnyauski S F.In vitro cellular ＆ developmental[J].Biol-ogy Plant,2001,37(4):427-433.

[18] Sandhu J S,Krasnyanski S F,Domier L L,et al.Oral immu-nization of mice with transgenie tomato fruit expressing respi-ratory syncytial virus-F protein induces a systemic immune response[J].Transgenie Res,2000,9(2):127-135.

[19] Yakoby N.Tobaceo ribosomal DNA spacer element elevates Bowman-Birk inhibitor expression in tomato plants[J].Plant Cell Rep,2006,25:573-581.

[20] 赵凌侠,金丽鑫,李柱刚,等.番茄E8启动子乙烯应答元件克隆及DNA序列分析[J].园艺学报,2004,31:204-205.

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