Abstract: Apair of primers(MAP301/MAP302)was designed based on the reported sequence of MAP30 in GenBank,and was then used to amplify the genomic DNA extracted from bitter melon by PCR,from which a 800 bp fragment was obtained.The fragment was cloned and then transformed to the E.coli DH5α after ligating with pMD18-T vector.The sequencing result of cloned fragment showed that it had 100% base similarity to the MAP30 gene.The cloned fragment was ligated with pEV1 or pEV2 vectors,and the expression vectors were construted after screening restriction analysis.The result provides the foundation for the further research on tomato as plant oral vaccine.

Key words: Bitter melon, MAP30, Construction of vector, PCR

CLC Number: 

• S642.50.6