Cloning of Arabidopsis thaliana DREB2A Gene and Its Construction of Its Plant Fluorescent Expression Vector

doi:10.7668/hbnxb.2008.03.006

Abstract

Abstract: In the paper,total RNA was extracted from Arabidcpsas thalaana seedlings and was used as template to amplify the DREB2A gene by RT-PCR.The gene fragment was subsequently cloned into plant expression vector pCAMBIA1304,located between CaMV35S promoter and poly(A)terminator.Restriction enzyme analysis and DNA sequencing confirmed that the plant expression vector pCAMBIA1304-DREB2A was successfully construed.In addition,the strain of Agrobacteraum twnefaciens carrying DREB2A gene were obtained,providing the foundation for transgenic study of plants.

Key words: Arabidcpsas thalaana, DREB2A, RT-PCR, PIant expression vector, Agrobacteraum twnefaciens

CLC Number:

DUAN Hong-ying, DING Xiao-sheng. Cloning of Arabidopsis thaliana DREB2A Gene and Its Construction of Its Plant Fluorescent Expression Vector[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(3): 20-22. doi: 10.7668/hbnxb.2008.03.006.

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