Isolation of Nitrogen Fixing Bacteria from Rhizosphere of Leymus chinensis and Investigation on their Growth Conditions

doi:10.3321/j.issn:1000-7091.2007.01.041

Abstract

Abstract: In order to get basic knowledge about abundance and distribution of N fixing bacteria in rhizosphere of Leymus chinensis in grassland, isolation of N fixing bacteria was conducted in midseason of Leymus chinensis growth in Xilinguole grassland of Inner Mongolia by culturing hydraulic extraction of soil and root samples in Doberiner's cultural media. The results showed that 14 strains of N fixing bacteria exist in the bulk soil, rhizosphere and roots of Leymus chinensis plants. Number of the N fixing bacteria in soil decreases gradually apart from Leymus chinensis plant roots. 4 strains of nitrogen-fixing bacteria with greater N fixing capacity from 14 strains, Y_c1 and Y_c2 isolated from roots and T_r1 and Tr₁₁ isolated from soil, were selected by comparing their nitrogenase activities for morphological observation and investigation of their growth condition. The results about growth condition of 4 strains of bacteria showed that they all can use sucrose and glucose very well, while they can not use cellulose at all. The best temperature for the 4 strains of bacteria growth is 25-35℃, and the two strains isolated from soil are more tolerant to both low temperature and high temperature than the ones from plant roots. Suitable pH is 7-8 for growth of the strains of N fixing bacteria.

Key words: Nitrogen-fixing bacteria, Isolation, Morphological characteristics, Growth condition

CLC Number:

S572.10

ZHENG Hong-li, PANG Bao-ping, FAN Ming-shou, LIU Shuang-ping. Isolation of Nitrogen Fixing Bacteria from Rhizosphere of Leymus chinensis and Investigation on their Growth Conditions[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(1): 172-177. doi: 10.3321/j.issn:1000-7091.2007.01.041.

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