Studies on the Technique of in planta Agrobacterium-mediated Gene Transfer by Vacuum Infiltration in Brassica juncea

doi:10.3321/j.issn:1000-7091.2004.03.003

Abstract

Abstract: In bud stage of mustards(Brassica juncea Coss) ecotype Xuelihong and Yuanyejie were trans-formed with in planta vacuum infiltration by an Agrobacterium tumefaciens strain GV3101 carrying hy-gromycin resistance gene. Effects of different factors on transformation frequency with in planta vacuum infil-tration were studied. The best transformation frequency was obtained in the following conditions, the vacuum infiltration time was 2 min/intermission 30s/30s，the concentration of Agrobacterium cells was ODboo 0.8，in-filtration medium was 1/2MS inorganic composition and BS organic composition, and in bud stage the buds were treated by the vacuum infiltration.

Key words: In planta vacuum infiltration, Genetic transformation, Agrobacterium tumefaciens, rassica uncea

CLC Number:

S634.4

JIN Wan-mei, GONG Zhen-hui, LU Yuan-hong. Studies on the Technique of in planta Agrobacterium-mediated Gene Transfer by Vacuum Infiltration in Brassica juncea[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2004, 19(3): 8-12. doi: 10.3321/j.issn:1000-7091.2004.03.003.

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