Dedifferentiation Behavior and Callus Regeneratibility of Wheat (Triticum aestivum L. ) Immature Embryos

doi:10.3321/j.issn:1000-7091.2003.01.018

Abstract

Abstract: Inves t igated in 30 cul t ivars(lines) of wheat, the morphologic inde xes are es tabl ished for wheat immature embryos sui table for in vi tro culture.Immature embryos were cul tured between 10 14 d after florescence in the pas t, but it was too extensive.The immature embryos are subt ransparent phase when young seed episperm becomes light green, raphe becomes hair and glos sless.This phase is just the t ime to cul ture immature embryos and only las ts for several hours.The genotype is very important for dedi fferentiation behaviors and cal lus re generatibili ty.

Key words: Wheat, Immature embryo, Tissue culture

CLC Number:

S512.1

LIU Shao xiang, WANG Hui, SUN Yi, LIANG Hong, CUI Gui mei. Dedifferentiation Behavior and Callus Regeneratibility of Wheat (Triticum aestivum L. ) Immature Embryos[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2003, 18(1): 64-67. doi: 10.3321/j.issn:1000-7091.2003.01.018.

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