Studies of Genetic Diversity in Maize Inbred Lines Based on SSRs and AFLPs Markers

doi:10.3321/j.issn:1000-7091.2003.01.017

Abstract

Abstract: The genetic diversi ty of 23 maize inbred lines and one te osinte accession was analyzed based on microsatelli te (SSR) and AFLPs markers. With 40 pai rs of SSR primers, total ly 202 polymorphic f ragments were detected. Twelve AFLP primer combina tion (Pst/Mse ) were used, and total ly 444 polymorphic bands were produced. BothSSRs and AFLPs were highly polymorphic. The average of polymorphic informat ion content (PIC) was 0. 6 for SSRs. Percentage of polymorphic AFLP bands was 72%.The resul ts of cluster analysis showed that the acces sions asses sed could be clustered into five groups in both marker sys tems. This is partly in accordance wih the previous classi fication based on conventional methods but wi th some dif ference. It was suggested that both sys tem can be used to analyze the genet ic diver sity of maize inbred lines.

Key words: Maize, SSR, AFLP, Genetic diversity

CLC Number:

S513

DU Jin you, LI Yu, WANG Tian yu, SHI Yun su, SONG Yan chun, WANG Hai bo. Studies of Genetic Diversity in Maize Inbred Lines Based on SSRs and AFLPs Markers[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2003, 18(1): 59-63. doi: 10.3321/j.issn:1000-7091.2003.01.017.

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